RT Journal Article
T1 Effective Actions of Ion Release from Mesoporous Bioactive Glass and Macrophage Mediators on the Differentiation of Osteoprogenitor and Endothelial Progenitor Cells
A1 Polo Montalvo, Alberto
A1 Casarrubios Molina, Laura
A1 Serrano, María Concepción
A1 Sanvicente, Adrián
A1 Feito Castellano, María José
A1 Arcos Navarrete, Daniel
A1 Portolés Pérez, María Teresa
AB Due to their specific mesoporous structure and large surface area, mesoporous bioactive glasses (MBGs) possess both drug-delivery ability and effective ionic release to promote bone regeneration by stimulating osteogenesis and angiogenesis. Macrophages secrete mediators that can affect both processes, depending on their phenotype. In this work, the action of ion release from MBG-75S, with a molar composition of 75SiO2-20CaO-5P2O5, on osteogenesis and angiogenesis and the modulatory role of macrophages have been assessed in vitro with MC3T3-E1 pre-osteoblasts and endothelial progenitor cells (EPCs) in monoculture and in coculture with RAW 264.7 macrophages. Ca2+, phosphorous, and silicon ions released from MBG-75S were measured in the culture medium during both differentiation processes. Alkaline phosphatase activity and matrix mineralization were quantified as the key markers of osteogenic differentiation in MC3T3-E1 cells. The expression of CD31, CD34, VEGFR2, eNOS, and vWF was evaluated to characterize the EPC differentiation into mature endothelial cells. Other cellular parameters analyzed included the cell size and complexity, intracellular calcium, and intracellular content of the reactive oxygen species. The results obtained indicate that the ions released by MBG-75S promote osteogenesis and angiogenesis in vitro, evidencing a macrophage inhibitory role in these processes and demonstrating the high potential of MBG-75S for the preparation of implants for bone regeneration.
PB MDPI
SN 1999-4923
YR 2021
FD 2021-07-27
LK https://hdl.handle.net/20.500.14352/4854
UL https://hdl.handle.net/20.500.14352/4854
LA eng
NO Ministerio de Economía y Competitividad (MINECO)/FEDER
DS Docta Complutense
RD 9 abr 2025