RT Journal Article
T1 Real-time correction of chromatic aberration in optical fluorescence microscopy
A1 Cayuela López, Ana
A1 Conesa, Pablo
A1 Oña Blanco, Ana María
A1 Gómez Pedrero, José Antonio
A1 Sánchez Sorzano, Carlos Oscar
AB Multi-color fluorescence imaging is a powerful tool for studying the spatial relationships and interactions among sub-cellular structures in biological specimens. However, if improperly corrected, geometrical distortions caused by mechanical drift, refractive index mismatch, or chromatic aberration can lead to lower image resolution. In this paper, we present an extension of the image processing framework of Scipion by integrating a protocol called OFM Corrector, which corrects geometrical distortions in real-time using a B-spline-based elastic continuous registration technique. Our proposal provides a simple strategy to overcome chromatic aberration by digitally re-aligning color channels in multi-color fluorescence microscopy images, even in 3D or time. Our method relies on a geometrical calibration, which we do with fluorescent beads excited by different wavelengths of light and subsequently registered to get the elastic warp as a reference to correct chromatic shift. Our software is freely available with a user-friendly GUI and can be broadly used for various biological imaging problems. The paper presents a valuable tool for researchers working in light microscopy facilities.
PB IOP Science
YR 2023
FD 2023
LK https://hdl.handle.net/20.500.14352/92911
UL https://hdl.handle.net/20.500.14352/92911
LA eng
NO Cayuela López, A., Conesa, P., Oña Blanco, A. M. et al. «Real-time correction of chromatic aberration in optical fluorescence microscopy». Methods and Applications in Fluorescence, vol. 11, n.o 4, octubre de 2023, p. 045001. DOI.org (Crossref), https://doi.org/10.1088/2050-6120/ace153.
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO European Commission
DS Docta Complutense
RD 29 may 2025