RT Journal Article
T1 Metabolomic characterization of MC3T3-E1pre-osteoblast differentiation induced by ipriflavone-loaded mesoporous nanospheres
A1 Casarrubios Molina, Laura
A1 Cicuéndez Maroto, Mónica
A1 Polo Montalvo, Alberto
A1 Feito Castellano, María José
A1 Martínez Del Pozo, Álvaro
A1 Arcos Navarrete, Daniel
A1 Duarte, Iola F.
A1 Portolés Pérez, María Teresa
AB This study reports on the metabolic changes accompanying the differentiation of MC3T3-E1 osteoprogenitor cells induced by mesoporous bioactive glass nanospheres (nMBG) loaded with ipriflavone (nMBG-IP). Ipriflavone (IP) is a synthetic isoflavone known for inhibiting bone resorption, maintaining bone density, and preventing osteoporosis. Delivering IP intracellularly is a promising strategy to modulate bone remodeling at significantly lower doses compared to free drug administration. Our results demonstrate that nMBG are efficiently internalized by pre-osteoblasts and, when loaded with IP, induce their differentiation. This differentiation process is accompanied by pronounced metabolic alterations, as monitored by NMR analysis of medium supernatants and cell extracts (exo- and endo-metabolomics, respectively). The main effects include an early-stage intensification of glycolysis and changes in several metabolic pathways, such as nucleobase metabolism, osmoregulatory and antioxidant pathways, and lipid metabolism. Notably, the metabolic impacts of nMBG-IP and free IP were very similar, whereas nMBG alone induced only mild changes in the intracellular metabolic profile without affecting the cells’ consumption/secretion patterns or lipid composition. This finding indicates that the observed effects are primarily related to IP-induced differentiation and that nMBG nanospheres serve as convenient carriers with both efficient internalization and minimal metabolic impact. Furthermore, the observed link between pre-osteoblast differentiation and metabolism underscores the potential of utilizing metabolites and metabolic reprogramming as strategies to modulate the osteogenic process, for instance, in the context of osteoporosis and other bone diseases.
PB Elsevier
SN 2772-9508
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/109869
UL https://hdl.handle.net/20.500.14352/109869
LA eng
NO Laura Casarrubios, Mónica Cicuéndez, Alberto Polo-Montalvo, María José Feito, Álvaro Martínez-del-Pozo, Daniel Arcos, Iola F. Duarte, María Teresa Portolés, Metabolomic characterization of MC3T3-E1pre-osteoblast differentiation induced by ipriflavone-loaded mesoporous nanospheres, Biomaterials Advances, Volume 166, 2025, 214085, https://doi.org/10.1016/j.bioadv.2024.214085.
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 24 abr 2025