RT Journal Article
T1 Expression of Human MDGA1 Increases Cell Motility and Cell-Cell Adhesion and Reduces Adhesion to Extracellular Matrix Proteins in MDCK Cells
A1 Díaz-López, Antonio
A1 Morán, Alberto
A1 Ortega, Paloma
A1 Iniesta Serrano, María Pilar
A1 Fernández Marcelo, Tamara
A1 Sánchez Pernaute, Andrés
A1 Torres García, Antonio José
A1 Benito De Las Heras, Manuel R.
A1 Juan Chocano, María Del Carmen De
AB Characterization of the novel human protein MDGA1 (MAM Domain containing Glycosylphosphatidylinositol Anchor-1) has been reported in our laboratory in the past few years. hMDGA1 is a glycoprotein containing 955 aminoacids (137kDa) attached to the eukaryotic cell membrane by a GPI (Glycosylphosphatidylinositol) anchor and localized specifically into membrane microdomains known as lipid rafts. Moreover, MDGA1 protein contains structural features found in different types of cell adhesion molecules (CAMs) such as the presence of immunoglobulin domains and a MAM domain (Meprin, A5 protein, receptor protein-tyrosine phosphatase mu), suggesting a role of MDGA1 in cell migration and/or adhesion. In order to investigate this aim, stable MDCK cell lines expressing MDGA1 or the truncated proteins IgGPI (lacking the MAM domain) and MAMGPI (lacking Ig domains) were generated. Our results reveal that MDGA1 increases the ability of MDCK cells to migrate, as it contains both Ig and MAM domains which have been implicated in cell motility. In addition, cell adhesion to extracellular matrix proteins, mainly to collagen IV, is reduced by MDGA1 and the IgGPI and MAMGPI truncated proteins. Accordingly, silencing MDGA1 by siRNA revealed a significant increase in adhesion to collagen IV. Furthermore, MDGA1 expression, through the intrinsic properties of the MAM domain, increases cell-cell adhesion independently of the cell monolayer used, suggesting that MDGA1 mediates cell-cell adhesiveness in a heterophilic manner.
PB Springer
SN 1875-2292
YR 2010
FD 2010-11-25
LK https://hdl.handle.net/20.500.14352/116213
UL https://hdl.handle.net/20.500.14352/116213
LA eng
NO Díaz-López A, Iniesta P, Morán A, Ortega P, Fernández-Marcelo T, Sánchez- Pernaute A, Torres AJ, Benito M, De Juan C. Expression of Human MDGA1 Increases Cell Motility and Cell-Cell Adhesion and Reduces Adhesion to Extracellular Matrix Proteins in MDCK Cells. Cancer Microenviron. 2010 Nov 25;4(1):23-32. doi: 10.1007/s12307-010-0055-2. PMID: 21505559; PMCID: PMC3047631.
DS Docta Complutense
RD 8 abr 2025