RT Journal Article
T1 Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for Neutrophil-Based Antitumour Treatment
A1 Bru Espino, Antonio Leonardo
A1 Souto Andres, Joan Carles
A1 Alcolea, Sonia
A1 Anton, Rosa
A1 Remacha, Angel
A1 Camacho, Mercedes
A1 Soler, Marta
A1 Bru, Isabel
A1 Porres, Amelia
A1 Vila, Luis
AB There is evidence that polymorphonuclear neutrophils (PMNs) can exert severe antineoplastic effects. Cross-talk between tumour cells and endothelial cells (ECs) is necessary for the accumulation of PMN around a tumour. This work reports the ability of two PMN-sensitive, human, permanent cell lines-colorectal adenocarcinoma (HT-29) and pharyngeal squamous-cell carcinoma (FaDu) cells-to act as inflammatory foci. PMNs were cytotoxic to both lines, the adhesion of the PMNs to the tumour cells being important in this effect. The tumour cells released appreciable amounts of IL-8 and GRO alpha, and induced the transmigration of PMN through human microvascular-EC monolayers. Conditioning media associated with both lines induced the adhesion of PMN and the surface expression of ICAM-1 in microvascular-EC. In addition, FaDu-conditioning-medium strongly induced the production of proinflammatory cytokines by microvascular-EC. These results support the idea that tumour cells might normally induce a potent acute inflammatory response, leading to their own destruction.
PB HINDAWI PUBLISHING CORPORATION
SN 0962-9351
YR 2009
FD 2009
LK https://hdl.handle.net/20.500.14352/42103
UL https://hdl.handle.net/20.500.14352/42103
LA eng
NO Bru Espino, A. L., Souto Andres, J. C., Alcolea, S. et al. «Tumour Cell Lines HT-29 and FaDu Produce Proinflammatory Cytokines and Activate Neutrophils In Vitro: Possible Applications for Neutrophil-Based Antitumour Treatment». Mediators of Inflammation, vol. 2009, 2009, pp. 1-13. DOI.org (Crossref), https://doi.org/10.1155/2009/817498.
DS Docta Complutense
RD 9 abr 2025