RT Journal Article
T1 Effect of Extender, Storage Time and Temperature on Kinetic Parameters (CASA) on Bull Semen Samples
A1 Fernandez-Novo, Aitor
A1 Santos López, Sergio
A1 Barrajon-Masa, Clara
A1 Mozas, Patricia
A1 de Mercado, Eduardo
A1 Caceres, Elisa
A1 Garrafa, Aizic
A1 González Martín, Juan Vicente
A1 Perez-Villalobos, Natividad
A1 Oliet, Agustín
A1 Astiz, Susana
A1 Perez-Garnelo, Sonia
AB CASA kinetic parameters are often evaluated in a diagnostic centre. How storage conditions affect ejaculates up to evaluation is unclear. We assessed, in 25 commercial bulls electroejaculated in the field, the impact of time until evaluation (0–2 h, 4–6 h, and 24 h post-ejaculation), holding temperature (5 ◦C vs. room temperature), and extender (AndroMed®, BIOXcell® or INRA96®) on CASA kinetic parameters. Total and progressive motility, VCL, VAP, VCL, ALH, BCF, STR, LIN, and WOB were assessed. CASA kinetic parameters were preserved for up to 4–6 h post-ejaculation, except for AndroMed®. Regardless of extender or temperature, motility decreased from 4–6 h up to 24 h, with the best values obtained with BIOXcell® at 5 ◦C. Our results suggest that BIOXcell® can preserve sperm motility for up to 6 h, either at 5 ◦C or room temperature, and also INRA96® at room temperature, with motility assessments and the percentage of the most rapid sperms being the lowest with INRA96® at 5 ◦C. The kinetic parameters decreased when analyses were performed at 24 h. Therefore, we suggest evaluating seminal quality as soon as possible, before 6 h after collection. These results help to fix adequate protocols for the short-term storage and shipment of bovine semen collected under field conditions.
PB MDPI
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/115873
UL https://hdl.handle.net/20.500.14352/115873
LA eng
NO Fernandez-Novo, A., Santos-Lopez, S., Barrajon-Masa, C., Mozas, P., de Mercado, E., Caceres, E., Garrafa, A., Gonzalez-Martin, J. V., Perez-Villalobos, N., Oliet, A., Astiz, S., & Perez-Garnelo, S. S. (2021). Effect of Extender, Storage Time and Temperature on Kinetic Parameters (CASA) on Bull Semen Samples. Biology, 10(8), 806. https://doi.org/10.3390/biology10080806
NO Conceptualization: A.F.-N., S.S.P.-G. and S.A.; methods: All authors; software: A.F.-N., S.S.-L., C.B.-M., P.M., A.G., A.O. and S.A.; validation: A.F.-N., S.S.P.-G. and S.A.; data analysis: A.F.-N., C.B.-M., P.M., E.d.M., E.C., A.G., A.O., S.S.P.-G. and S.A.; investigation: A.F.-N., S.S.P.-G. and S.A.; resources: A.F.-N., S.S.-L., A.G., A.O., S.S.P.-G. and S.A.; data curation: A.F.-N., E.d.M., E.C., J.V.G.-M. and S.A.; drafting of the manuscript: A.F.-N., S.S.P.-G. and S.A.; review and editing of the manuscript: All authors.; supervision: A.F.-N., S.S.P.-G. and S.A.; project administration: S.S.P.-G. and S.A. All authors have read and agreed to the published version of the manuscript.
NO Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)
DS Docta Complutense
RD 8 abr 2025