RT Journal Article
T1 CLytA-DAAO, free and immobilized in magnetic nanoparticles, induces cell death in human cancer cells
A1 Fuentes-Baile, María
A1 Bello-Gil, Daniel
A1 Pérez-Valenciano, Elizabeth
A1 Sanz, Jesús
A1 García-Morales, Pilar
A1 Maestro García-Donas, María Beatriz
A1 Ventero, María
A1 Alenda, Cristina
A1 Barberá, Victor
A1 Saceda, Manuel
AB D-amino acid oxidase (DAAO) catalyzes the oxidation of D-amino acids generating hydrogen peroxide, a potential producer of reactive oxygen species. In this study, we used a CLytA-DAAO chimera, both free and bound to magnetic nanoparticles, against colon carcinoma, pancreatic adenocarcinoma, and glioblastoma cell lines. We found that the enzyme induces cell death in most of the cell lines tested and its efficiency increases significantly when it is immobilized in nanoparticles. We also tested this enzyme therapy in non-tumor cells, and we found that there is not cell death induction, or it is significantly lower than in tumor cells. The mechanism triggering cell death is apparently a classical apoptosis pathway in the glioblastoma cell lines, while in colon and pancreatic carcinoma cell lines, CLytA-DAAO-induced cell death is a necrosis. Our results constitute a proof of concept that an enzymatic therapy, based on magnetic nanoparticles-delivering CLytA-DAAO, could constitute a useful therapy against cancer and besides it could be used as an enhancer of other treatments such as epigenetic therapy, radiotherapy, and treatments based on DNA repair.
SN 2218-273X
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/94062
UL https://hdl.handle.net/20.500.14352/94062
LA eng
NO Fuentes-Baile, M.; Bello-Gil, D.; Pérez-Valenciano, E.; Sanz, J.M.; García-Morales, P.; Maestro, B.; Ventero, M.P.; Alenda, C.; Barberá, V.M.; Saceda, M. CLytA-DAAO, Free and Immobilized in Magnetic Nanoparticles, Induces Cell Death in Human Cancer Cells. Biomolecules 2020, 10, 222. https://doi.org/10.3390/biom10020222
NO Instituto de Salud Carlos III
NO Consejo Superior de Investigaciones Científicas
DS Docta Complutense
RD 8 abr 2025