RT Journal Article
T1 Strategies to Reduce the Use of Antibiotics in Fresh and Chilled Equine Semen
A1 Zabala Argüelles, Sonsoles Mercedes
A1 Serres Dalmau, María Consolacion
A1 Montero Serra, Natalia
A1 Crespo Castejón, Francisco
A1 Lorenzo González, Pedro Luis
A1 Pérez-Aguilera, Verónica
A1 Galán López, Carmen
A1 Domínguez Gimbernat, Mónica
A1 Oliet Palá, Agustín
A1 Moreno, Santiago
A1 González Zorn, Bruno
A1 Gutiérrez Cepeda, Luna
AB The study assessed the impact of four equine semen processing techniques on sperm quality and microbial load immediately post-processing and after 48 h of refrigeration. The aim was to explore the potential reduction of prophylactic antibiotic usage in semen extenders. Semen from ten adult stallions was collected and processed under a strict hygiene protocol and divided into four aliquots: Simple Centrifugation with antibiotics (SC+), Simple Centrifugation (SC−), Single-Layer Colloidal Centrifugation (CC−), and Filtration (with SpermFilter®) (F−), all in extenders without antibiotics. Sperm motility, viability, and microbial load on three culture media were assessed. No significant differences were observed in the main in the sperm quality parameters among the four protocols post-processing and at 48 h (p < 0.05 or p < 0.1). Microbial loads in Columbia 5% Sheep Blood Agar and Schaedler vitamin K1 5% Sheep Blood Agar mediums were significantly higher (p < 0.10) for raw semen than for CS+, CC−, and F− post-processing. For Sabouraud Dextrose Agar medium, the microbial load was significantly higher (p < 0.10) in raw semen compared to CS+ and F−. No significant differences (p < 0.10) were found in 48 h chilled samples. Regardless of antibiotic presence, the evaluated processing methods, when combined with rigorous hygiene measures, maintained semen quality and reduced microbial load to the same extent as a traditional protocol using antibiotics.
PB MDPI
SN 2076-2615
YR 2024
FD 2024-01-05
LK https://hdl.handle.net/20.500.14352/102498
UL https://hdl.handle.net/20.500.14352/102498
LA eng
NO Zabala SM, Serres C, Montero N, Crespo F, Lorenzo PL, Perez-Aguilera V, Galan C, Dominguez-Gimbernat M, Oliet A, Moreno S, Gonzalez-Zorn B and Gutierrez-Cepeda L*. Strategies to Reduce the Use of Antibiotics in Fresh and Chilled Equine Semen. Animals, 14(2):179. 2024. (A). ISSN: 2076-2615. Impact factor 2022: 3.000. Category: Agriculture, dairy & animal science, Quartile: 1, Position: 12 of 62. DOI: 10.3390/ani14020179
NO Author Contributions: The study was conceived by L.G.-C. and C.S. who, together with S.M.Z., B.G.-Z., P.L.L., S.M. and F.C., participated in its design and coordination. S.M.Z., L.G.-C., V.P.-A., M.D.-G., C.G. and F.C. performed the semen collection, and processed the samples for refrigeration. Semen evaluation was executed by S.M.Z., V.P.-A., A.O., C.G., M.D.-G., F.C. and L.G.-C. Microbial load assessment was conducted by B.G.-Z. and N.M. with the assistance of S.M.Z. and V.P.-A. for sample preparation. L.G.-C. and C.S. performed the statistical analysis, with the support of Pedro Cuesta. C.S., L.G.-C., S.M.Z., B.G.-Z., P.L.L., F.C. and S.M. interpreted the data. The manuscript was written by L.G.-C., S.M.Z., C.S. and F.C., helped by the rest of authors, who revised it critically. All authors have read and approved the final version of the manuscript and agree to be accountable for all aspects of the work in ensuring that questions related to the accuracy or integrity of any part of the work have been appropriately investigated and resolved. All authors have read and agreed to the published version of the manuscript.
NO Instituto Madrileño de Investigación y Desarrollo Rural, Agrario y Alimentario
DS Docta Complutense
RD 18 jul 2024