RT Journal Article
T1 Metabolomics of meat exudate: Its potential to evaluate beef meat conservation and aging
A1 Castejón, David
A1 García Segura, Juan Manuel
A1 Escudero Portugués, Rosa María
A1 Herrera Fernández, Antonio
A1 Cambero Rodríguez, María Isabel
AB In this study we analyzed the exudate of beef to evaluate its potential as non invasive sampling for nuclear magnetic resonance (NMR) based metabolomic analysis of meat samples. Exudate, as the natural juice from raw meat, is an easy to obtain matrix that it is usually collected in small amounts in commercial meat packages. Although meat exudate could provide complete and homogeneous metabolic information about the whole meat piece, this sample has been poorly studied. Exudates from 48 beef samples of different breeds, cattle and storage times have been studied by 1H NMR spectroscopy. The liquid exudate spectra were compared with those obtained by High Resolution Magic Angle Spinning (HRMAS) of the original meat pieces. The close correlation found between both spectra (>95% of coincident peaks in both registers; Spearman correlation coefficient = 0.945) lead us to propose the exudate as an excellent alternative analytical matrix with a view to apply meat metabolomics. 60 metabolites could be identified through the analysis of mono and bidimensional exudate spectra, 23 of them for the first time in NMR meat studies. The application of chemometric tools to analyze exudate dataset has revealed significant metabolite variations associated with meat aging. Hence, NMR based metabolomics have made it possible both to classify meat samples according to their storage time through Principal Component Analysis (PCA), and to predict that storage time through Partial Least Squares (PLS) regression.
PB Elsevier
SN 0003-2670
YR 2015
FD 2015-12-11
LK https://hdl.handle.net/20.500.14352/23373
UL https://hdl.handle.net/20.500.14352/23373
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
DS Docta Complutense
RD 22 abr 2026