RT Journal Article
T1 In vitro interference of β-lactams with biofilm development by prevalent community respiratory tract isolates
A1 Maestre, Juan Ramón
A1 Mateo, María
A1 Méndez, María Luisa
A1 Aguilar, Lorenzo
A1 Gimenez, María José
A1 Alou Cervera, Luis
A1 Coronel, Pilar
A1 Granizo, Juan José
A1 Prieto Prieto, José
AB Interference of cefditoren (CDN) and amoxicillin/clavulanic acid (AMC) with biofilm production was studied using 11 Streptococcus pneumoniae isolates with minimum inhibitory concentrations (MICs) ranging from 0.015microg/mL to 0.5microg/mL for CDN and from 0.06microg/mL to 2microg/mL for AMC (except for one isolate with an AMC MIC of 8microg/mL) and 5 Haemophilus influenzae isolates with MICs of 0.03-0.06microg/mL for CDN and 0.5-16microg/mL for AMC. Slime production was assessed in antibiotic-free medium and with 0.03microg/mL CDN or 1/0.5microg/mL AMC by measuring the optical density at 450nm (OD(450)). Significantly lower mean OD(450) values were obtained for S. pneumoniae with antibiotics compared with controls (CDN, 0.088 vs. 0.118, P=0.003; and AMC, 0.095 vs. 0.112, P=0.003), with significant correlation between both antibiotics (r=0.752; P=0.008). Percent reduction in OD(450) values was higher for CDN compared with AMC (24.02% vs. 15.92%; P=0.008). For H. influenzae, significantly lower mean OD(450) values were obtained with CDN compared with controls (0.083 vs. 0.096; P=0.043) but not with AMC (0.086 vs. 0.095; P=0.08). Comparing percent reductions in S. pneumoniae versus H. influenzae for each antibiotic, no differences were found for AMC (15.92% vs. 9.40%; P=0.36), with a tendency for CDN (24.02% vs. 13.79%; P=0.069). Different beta-lactams may have different capabilities of interfering with S. pneumoniae biofilm development when tested under the same experimental conditions.
PB Elsevier
SN 0924-8579
YR 2010
FD 2010-03
LK https://hdl.handle.net/20.500.14352/107047
UL https://hdl.handle.net/20.500.14352/107047
LA eng
NO Maestre JR, Mateo M, Méndez ML, Aguilar L, Gimenez MJ, Alou L, Coronel P, Granizo JJ, Prieto J. In vitro interference of beta-lactams with biofilm development by prevalent community respiratory tract isolates. Int J Antimicrob Agents. 2010 Mar;35(3):274-7.
NO Tedec-Meiji Farma S.A.
DS Docta Complutense
RD 25 abr 2025