RT Journal Article
T1 Long chain fatty acids block allergic reaction against lipid transfer protein Sola l 7 from tomato seeds
A1 Parrón Ballesteros, Jorge
A1 Martín-Pedraza, Laura
A1 García Gordo, Rubén
A1 Mayorga, Cristobalina
A1 Pastor Vargas, Carlos
A1 Titaux-Delgado, Gustavo A.
A1 Villalba, Mayte
A1 Batanero, Eva
A1 Pantoja-Uceda, David
A1 Turnay, Javier
AB Due to the benefits of tomato as an antioxidant and vitamin source, allergy to this vegetable food is a clinical concerning problem. Sola l 7, a class I Lipid Transfer Protein found in tomato seeds, has been identified as an allergen linked to severe anaphylaxis. However, the role of lipid binding in Sola l 7 induced allergy remains unclear. Here, the three-dimensional structure of recombinant Sola l 7 (rSola l 7) has been elucidated using nuclear magnetic resonance spectroscopy (NMR). Its interaction with free fatty acids has been deeply studied; fluorescence emission spectroscopy revealed that different long-chain fatty acids interact with the protein affecting the only tyrosine residue present in Sola l 7. On the contrary, no changes in the overall secondary structure were observed after the analysis of the circular dichroism spectra in the presence of fatty acids. Unsaturated oleic and linoleic fatty acids presented higher affinity and promoted more significant changes than saturated or short chain fatty acids. 1H-15N HSQC NMR spectra allowed to determine the regions of the protein that were modified when rSola l 7 interacts with the fatty acids, suggesting epitope modification after the interaction. For corroboration, IgG and IgE binding to rSola l 7 were assessed in the presence of free fatty acids, revealing that both IgE and IgG binding was significantly lower than in their absence, suggesting a potential protective role of unsaturated fatty acids in tomato allergy.
PB Wiley
YR 2024
FD 2024-08-09
LK https://hdl.handle.net/20.500.14352/109241
UL https://hdl.handle.net/20.500.14352/109241
LA eng
NO Parrón-Ballesteros J, Martín-Pedraza L, Gordo RG, Mayorga C, Pastor-Vargas C, Titaux-Delgado GA, Villalba M, Batanero E, Pantoja-Uceda D, Turnay J. Longchain fatty acids block allergic reaction against lipid transfer protein Sola l 7 from tomato seeds. Protein Science. 2024;33(9):e5154
NO Ministerio de Ciencia e Innovaci on
NO Banco Santander
NO Servicio Andaluz de Salud
DS Docta Complutense
RD 8 abr 2025