RT Journal Article
T1 Serum protein electrophoresis in European mink (Mustela lutreola): reference intervals and comparison of agarose gel electrophoresis and capillary zone electrophoresis
A1 Villanueva Saz, Sergio
A1 Aranda, María del Carmen
A1 Jiménez Martínez, María De Los Ángeles
A1 Andrés Gamazo, Paloma Jimena De
A1 Verde, Maite
A1 Climent, María
A1 Lebrero Berna, María Eugenia
A1 Marteles Aragüés, Diana
A1 Fernández, Antonio
AB Background: Knowledge of reference intervals for blood analytes, including serum protein fractions, is of great importance for the identification of infectious and inflammatory diseases and is often lacking in wild animal species.Material and methods: Serum samples were obtained from European minks enrolled in the breeding program (n = 55). Agarose gel electrophoresis (AGE) and capillary zone electrophoresis (CZE) were used to separate and identify protein fractions. Albumin, α1, α2, β, and γ-globulins fractions were identified in all mink sera by both electrophoresis methods. Reference intervals (90% CI) were determined following the 2008 guidelines of the Clinical Laboratory Standard Institute. The methods were compared using Passing-Bablok regression, Bland–Altman analysis, and Lin’s concordance correlation.Results: A significant bias was found between methods for α1, α2, and γ-globulin. Lin’s concordance correlation was considered unacceptable for α1, α2, and β-globulins. Differences for gender between methods were found for albumin and α2-globuins, which were higher for males than females. γ-globulins were higher for adults than young minks using both methods; however, α1 and α2-globulins were lower.Conclusion: Both methods are adequate for identifying serum protein disorders, but the AGE and CZE methods are not equivalent. Therefore, reference intervals for each technique are required.
PB Taylor & Francis
SN 0165-2176
YR 2024
FD 2024-02-22
LK https://hdl.handle.net/20.500.14352/103341
UL https://hdl.handle.net/20.500.14352/103341
LA eng
DS Docta Complutense
RD 25 ago 2024