RT Journal Article
T1 TaqMan real-time PCR assay for detection of traces of Brazil nut (Bertholletia excelsa) in food products
A1 Cruz, Silvia de la
A1 López-Calleja, Inés María
A1 Alcocer, Marcos
A1 González Alonso, María Isabel
A1 Martín De Santos, María Del Rosario
A1 García Lacarra, Teresa
AB Mislabeling of food products containing Brazil nut may represent a serious thread for allergic consumers. In order to protect sensitized individuals, reliable methods to detect trace amounts of Brazil nut must be accessible to food industry as well as Food Safety authorities. According to this, a TaqMan real-time polymerase chain reaction (PCR) method has been developed for specific detection of Brazil nut in foodstuffs. The method employs Brazil nut specific primers and probe, targeting 2S albumin gene (Ber e 1), and a positive amplification control based on 18S rRNA gene. Results obtained on sensitivity with wheat flour spiked with different concentrations of raw and heat treated Brazil nut showed that the limit of detection (LOD) for the technique was 2.5 mg/kg. Applicability of the Brazil nut specific system was assessed through analysis of 66 different commercial food samples. The reported real-time PCR assay provides a useful tool for detection of Brazil nut DNA, and it can be used as a routine analysis to assert accuracy on food labeling.
PB Elsevier
SN 0956-7135
YR 2013
FD 2013
LK https://hdl.handle.net/20.500.14352/94200
UL https://hdl.handle.net/20.500.14352/94200
LA eng
NO De La Cruz, S., López-Calleja, I., Alcocer, M., González, I., Martı́n, R., & Garcı́a, T. (2013). TAQMAN Real-time PCR assay for detection of traces of Brazil nut (Bertholletia excelsa) in food products. Food Control, 33(1), 105-113. https://doi.org/10.1016/j.foodcont.2013.01.053
NO Comunidad de Madrid
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 6 oct 2024