RT Journal Article
T1 Towards Control and Oversight of SARS-CoV-2 Diagnosis andMonitoring through Multiplexed Quantitative ElectroanalyticalImmune Response Biosensors
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Montero Calle, Ana
A1 San Bartolomé, Clara
A1 Cano, Olga
A1 Vazquez, Monica
A1 Iglesias Caballero, María
A1 Corral Lugo, Andrés
A1 McConnell, Michael J.
A1 Pascal, Mariona
A1 Mas, Vicente
A1 Pingarrón Carrazón, José Manuel
A1 Barderas Manchado, Rodrigo
A1 Campuzano Ruiz, Susana
AB The development of versatile and sensitive biotools to quantify specific SARS-CoV-2 immunoglobulins in SARS-CoV-2 infected and non-infected individuals, built-on the surface of magnetic microbeads functionalized with nucleocapsid (N) and inhouse expressed recombinant spike (S) proteins is reported.Amperometric interrogation of captured N- and S-specific circulating total or individual immunoglobulin (Ig) isotypes (IgG, IgM, and IgA), subsequently labelled with HRP-conjugated secondary antibodies, was performed at disposable single or multiplexed (8) screen-printed electrodes using the HQ/HRP/H2O2 system. The obtained results using N and in-house expressed S ectodomains of five SARS-CoV-2 variants of concern (including the latest Delta and Omicron) allow identification of vulnerable populations from those with natural or acquired immunity, monitoring of infection, evaluation of vaccine efficiency and even identification of the variant responsible for the infection.
PB Wiley
SN 1433-7851
YR 2022
FD 2022-05-04
LK https://hdl.handle.net/20.500.14352/71437
UL https://hdl.handle.net/20.500.14352/71437
LA eng
NO CRUE-CSIC (Acuerdos Transformativos 2022)
NO Ministerio de Ciencia e Innovación (MICINN)
NO Comunidad de Madrid
NO Instituto de Salud Carlos III (ISCIII)
DS Docta Complutense
RD 6 abr 2025