RT Journal Article
T1 Full enzymatic hydrolysis of commercial sucrose laurate by immobilized-stabilized derivatives of lipase from Thermomyces lanuginosa
A1 Marciello, Marzia
A1 Mateo González, César
A1 Guisan Seijas, José Manuel
AB Sucrose laurate is a detergent that is useful for various biochemical applications because it is a green compound and is easily degradable after hydrolysis with a lipase or esterase. One problem observed in the process of sucrose laurate degradation is that most commercial detergent preparations are impure, necessitating the hydrolysis of all of the sucrose esters present in the preparation, all of them with detergent properties. In this article, a highly active catalyst, which is able to perform the hydrolysis of commercial sucrose laurate, is presented. The use of glyoxyl agarose preparations of a previously aminated Thermomyces lanuginosa lipase (TLL) enabled complete hydrolysis, in less than 30 min, of all of the compounds that comprise the mixture. In addition, this derivative is stable in the presence of 20% ethanol, which is necessary to prevent microbial contamination.
PB Elsevier
SN 0927-7765
YR 2011
FD 2011-02-16
LK https://hdl.handle.net/20.500.14352/100870
UL https://hdl.handle.net/20.500.14352/100870
LA eng
NO Marciello M, Mateo C, Guisan JM. Full enzymatic hydrolysis of commercial sucrose laurate by immobilized-stabilized derivatives of lipase from Thermomyces lanuginosa. Colloids and Surfaces B: Biointerfaces 2011;84:556–60. https://doi.org/10.1016/j.colsurfb.2011.02.017.
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Pharmamar S.A.
NO Biotecnologías Aplicadas
DS Docta Complutense
RD 18 abr 2025