RT Journal Article
T1 Conformational and biochemical differences in the TCR/CD3 complex of CD8+ vs CD4+ mature lymphocytes revealed in the absence of CD3γ
A1 Zapata, David A.
A1 Pacheco Castro, Alberto
A1 Torres, Pilar S.
A1 Ramiro, Almudena R.
A1 San José, Ester
A1 Alarcón, Balbino
A1 Alibaud, Laeticia
A1 Rubin, Bent
A1 Toribio, María L.
A1 Regueiro González-Barros, José Ramón
AB Mature CD4(+) and CD8(+) T lymphocytes are believed to build and express essentially identical surface alphabeta T-cell receptor-CD3 (TCR.CD3) complexes. However, TCR.CD3 expression has been shown to be more impaired in CD8(+) cells than in CD4(+) cells when CD3gamma is absent in humans or mice. We have addressed this paradox by performing a detailed phenotypical and biochemical analysis of the TCR.CD3 complex in human CD3gamma-deficient CD8(+) and CD4(+) T cells. The results indicated that the membrane TCR.CD3 complex of CD8(+) T lymphocytes was conformationally different from that of CD4(+) lymphocytes in the absence of CD3gamma. In addition, CD8(+), but not CD4(+), CD3gamma-deficient T lymphocytes were shown to contain abnormally glycosylated TCRbeta proteins, together with a smaller, abnormal TCR chain (probably incompletely processed TCRalpha). These results suggest the existence of hitherto unrecognized biochemical differences between mature CD4(+) and CD8(+) T lymphocytes in the intracellular control of alphabetaTCR. CD3 assembly, maturation, or transport that are revealed when CD3gamma is absent. Such lineage-specific differences may be important in receptor-coreceptor interactions during antigen recognition.
PB Elsevier
SN 0021-9258
YR 1999
FD 1999-08-27
LK https://hdl.handle.net/20.500.14352/107334
UL https://hdl.handle.net/20.500.14352/107334
LA eng
NO Zapata DA, Pacheco-Castro A, Torres PS, Ramiro AR, San José E, Alarcón B, Alibaud L, Rubin B, Toribio ML, Regueiro JR. Conformational and biochemical differences in the TCR.CD3 complex of CD8(+) versus CD4(+) mature lymphocytes revealed in the absence of CD3gamma. J Biol Chem. 1999 Dec 3;274(49):35119-28. doi: 10.1074/jbc.274.49.35119. PMID: 10574994.
NO Ministerio de Educación y Cultura (España)
NO Comunidad Autónoma de Madrid
NO Comisión Interministerial de Ciencia y Tecnología (España)
NO Dirección General de Ensenanza Superior e Investigación Científica (España)
NO Ministerio de Educación y Cultura (España)
DS Docta Complutense
RD 6 abr 2025