RT Journal Article
T1 Novel Use of PLGA Microspheres to Create an Animal Model of Glaucoma with Progressive Neuroretinal Degeneration
A1 García Herranz, David
A1 Rodrigo, Maria Jesus
A1 Subias, Manuel
A1 Martínez Rincón, Teresa
A1 Méndez Martínez, Silvia
A1 Bravo Osuna, Irene
A1 Bonet, Aina
A1 Ruberte, Jesus
A1 García Feijoo, Julián
A1 García Martín, Elena Salobrar
A1 Pablo, Luis
A1 Herrero Vanrell, María Del Rocío
AB Progressive degeneration of neuroretinal tissue with maintained elevated intraocular pressure (IOP) to simulate chronic glaucoma was produced by intracameral injections of poly (lactic-co-glycolic) acid (PLGA) microspheres (Ms) in rat eyes. The right eye of 39 rats received different sizes of PLGA-Ms (2 µL suspension; 10% w/v): 14 with 38–20 µm Ms (Ms38/20 model) and 25 with 20–10 µm particles (Ms20/10 model). This novel glaucoma animal model was compared to the episcleral vein sclerosis (EPI) model (25 eyes). Injections were performed at baseline, two, four and six weeks. Clinical signs, IOP, retina and optic nerve thicknesses (using in vivo optical coherence tomography; OCT), and histological studies were performed. An IOP increment was observed in all three groups, however, the values obtained from the PLGA-Ms injection resulted lower with a better preservation of the ocular surface. In fact, the injection of Ms20/10 created a gentler, more progressive, and more sustained increase in IOP. This IOP alteration was correlated with a significant decrease in most OCT parameters and in histological ganglion-cell count for the three conditions throughout the eight-week follow-up. In all cases, progressive degeneration of the retina, retinal ganglion cells and optic nerve, simulating chronic glaucoma, was detected by OCT and corroborated by histological study. Results showed an alternative glaucoma model to the well-known episcleral vein model, which was simpler to perform, more reproducible and easier to monitor in vivo.
PB MDPI
SN 1999-4923
YR 2021
FD 2021-02-08
LK https://hdl.handle.net/20.500.14352/7070
UL https://hdl.handle.net/20.500.14352/7070
LA eng
NO Ministerio de Economía, Comercio y Empresa (España)/Fondo Europeo de Desarrollo Regional
NO Instituto de Salud Carlos III/Fondo Europeo de Desarrollo Regional
NO Instituto de Salud Carlos III
NO Universidad Complutense de Madrid/Banco de Santander
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 22 abr 2025