%0 Journal Article
%A Mejías, Raquel
%A Costo, Rocío
%A Roca, Alejandro G.
%A Fernández Arias, Cristina
%A Veintemillas-Verdaguer, S.
%A González-Carreño, T.
%A Puerto Morales, M. del
%A Serna, C.J.
%A Mañes, S.
%A Barber, D.F.
%T Cytokine adsorption/release on uniform magnetic nanoparticles for localized drug delivery
%D 2008
%@ 0168-3659
%U https://hdl.handle.net/20.500.14352/96469
%X Attachment of cytokines to magnetic nanoparticles has been developed as a system for controlled local drug release in cancer therapy. We studied the adsorption/release of murine interferon gamma (IFN-gamma) on negatively charged magnetic nanoparticles prepared by three different methods, including coprecipitation, decomposition in organic media, and laser pyrolysis. To facilitate IFN-gamma adsorption, magnetic nanoparticles were surface modified by distinct molecules to achieve high negative charge at pH 7, maintaining small aggregate size and stability in biological media. We analyzed carboxylate-based coatings and studied the colloidal properties of the resulting dispersions. Finally, we incubated the magnetic dispersions with IFN-gamma and determined optimal conditions for protein adsorption onto the particles, as well as the release capacity at different pH and as a function of time. Particles prepared by decomposition in organic media and further modified with dimercaptosuccinic acid showed the most efficient adsorption/release capacity. IFN-gamma adsorbed on these nanoparticles would allow concentration of this protein or other biomolecules at specific sites for treatment of cancer or other diseases.
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