RT Journal Article
T1 Cytokine adsorption/release on uniform magnetic nanoparticles for localized drug delivery
A1 Mejías, Raquel
A1 Costo, Rocío
A1 Roca, Alejandro G.
A1 Fernández Arias, Cristina
A1 Veintemillas-Verdaguer, S.
A1 González-Carreño, T.
A1 Puerto Morales, M. del
A1 Serna, C.J.
A1 Mañes, S.
A1 Barber, D.F.
AB Attachment of cytokines to magnetic nanoparticles has been developed as a system for controlled local drug release in cancer therapy. We studied the adsorption/release of murine interferon gamma (IFN-gamma) on negatively charged magnetic nanoparticles prepared by three different methods, including coprecipitation, decomposition in organic media, and laser pyrolysis. To facilitate IFN-gamma adsorption, magnetic nanoparticles were surface modified by distinct molecules to achieve high negative charge at pH 7, maintaining small aggregate size and stability in biological media. We analyzed carboxylate-based coatings and studied the colloidal properties of the resulting dispersions. Finally, we incubated the magnetic dispersions with IFN-gamma and determined optimal conditions for protein adsorption onto the particles, as well as the release capacity at different pH and as a function of time. Particles prepared by decomposition in organic media and further modified with dimercaptosuccinic acid showed the most efficient adsorption/release capacity. IFN-gamma adsorbed on these nanoparticles would allow concentration of this protein or other biomolecules at specific sites for treatment of cancer or other diseases.
PB Elsevier
SN 0168-3659
YR 2008
FD 2008-09-10
LK https://hdl.handle.net/20.500.14352/96469
UL https://hdl.handle.net/20.500.14352/96469
LA eng
DS Docta Complutense
RD 12 abr 2025