RT Journal Article
T1 Garvicins AG1 and AG2: Two Novel Class IId Bacteriocins of Lactococcus garvieae Lg-Granada
A1 Maldonado Barragán, Antonio
A1 Alegría Carrasco, Estíbaliz
A1 Blanco, María del Mar
A1 Vela Alonso, Ana Isabel
A1 Rodríguez, Juan Miguel
A1 Gibello Prieto, Alicia
A1 Fernández-Garayzábal Fernández, José Francisco
AB Lactococcus garvieae causes infectious diseases in animals and is considered an emerging zoonotic pathogen involved in human clinical conditions. In silico analysis of plasmid pLG50 of L. garvieae Lg-Granada, an isolate from a patient with endocarditis, revealed the presence of two gene clusters (orf46–47 and orf48–49), each one encoding a novel putative bacteriocin, i.e., garvicin AG1 (GarAG1; orf46) and garvicin AG2 (GarAG2; orf48), and their corresponding immunity proteins (orf47 and orf49). The chemically synthesised bacteriocins GarAG1 and GarAG2 presented inhibitory activity against pathogenic L. garvieae strains, with AG2 also being active against Listeria monocytogenes, Listeria ivanovii and Enterococcus faecalis. Genetic organisation, amino acid sequences and antimicrobial activities of GarAG1 and GarAG2 indicate that they belong to linear non-pediocin-like one-peptide class IId bacteriocins. Gram-positive bacteria that were sensitive to GarAG2 were also able to ferment mannose, suggesting that this bacteriocin could use the mannose phosphotransferase transport system (Man-PTS) involved in mannose uptake as a receptor in sensitive strains. Intriguingly, GarAG1 and GarAG2 were highly active against their own host, L. garvieae Lg-Granada, which could be envisaged as a new strategy to combat pathogens via their own weapons.
PB MPDI
SN 1422-0067
YR 2022
FD 2022-04-23
LK https://hdl.handle.net/20.500.14352/73222
UL https://hdl.handle.net/20.500.14352/73222
LA eng
NO Ministerio de Ciencia, Innovación y Universidades (MCIU)
DS Docta Complutense
RD 6 abr 2025