RT Journal Article
T1 Endocannabinoid-Hydrolysing Enzymes in the Post-Mortem Cerebellum of Humans Affected by Hereditary Autosomal Dominant Ataxias
A1 Romero, Julián
A1 Hernández Gálvez, María Luz
A1 Rodríguez Cueto, Carmen Aurora
A1 Fernández Ruiz, José Javier
A1 Benito Villalvilla, Cristina
A1 Gómez Ruiz, María Sagrario
AB Objectives: Spinocerebellar ataxias (SCAs) are characterized by a loss of balance and motor coordination due to degeneration of the cerebellum and its afferent and efferent connections. We recently found important changes in cannabinoid CB1 and CB2 receptors in the post-mortem cerebellum of patients affected by different SCAs.Methods: We wanted to further explore this issue by analysing the two major endocannabinoid-hydrolysing enzymes, fatty acid amide hydrolase (FAAH) and monoacyl glycerol lipase (MAGL), in the post-mortem cerebellum of SCA patients and control subjects.Results: Immunoreactivity for the FAAH and MAGL enzymes was found in the granular layer, in Purkinje cells, in neurons of the dentate nucleus and in areas of white matter in the cerebellum of patients at levels frequently notably higher than those in control subjects. Using double-labelling procedures, we found co-localization of FAAH and MAGL with calbindin, supporting the presence of these enzymes in Purkinje neurons.Conclusions: Degradative endocannabinoid enzymes are significantly increased in the cerebellum of SCA patients, which would presumably lead to reduced endocannabinoid levels. The identification of these enzymes in Purkinje neurons suggests a relationship with the pathogenesis of SCAs and suggests that the endocannabinoid system could provide potential therapeutic targets for the treatment of disease progression in SCAs.
PB Karger Publishers
SN 1015-2008
SN 1423-0291
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/92905
UL https://hdl.handle.net/20.500.14352/92905
LA eng
NO Rodríguez-Cueto C, Benito C, Romero J, Hernández-Gálvez M, Gómez-Ruiz M, Fernández-Ruiz J. Endocannabinoid-hydrolysing enzymes in the post-mortem cerebellum of humans affected by hereditary autosomal dominant ataxias. Pathobiology. 2014;81(3):149-59. doi: 10.1159/000358127. Epub 2014 Mar 14. PMID: 24642775.
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas
NO Fundación Eugenio Rodríguez Pascual
DS Docta Complutense
RD 27 abr 2025