%0 Journal Article
%A Muro, Manuel
%A Sánchez-Bueno, Francisco
%A Marín, Luis Alberto
%A Torío, Alberto
%A Moya-Quiles, María Rosa
%A Minguela, Alfredo
%A Montes, Olga
%A Guerra Pérez, Natalia
%A Arana Montes, María Del Pilar De
%A Pérez-López, Maria José
%A Robles, Ricardo
%A Ramirez, Pablo
%A García-Alonso, Ana María
%A Parrilla, Pacual
%A Álvarez-López, María Rocio
%T Relationship between CDC cross-match in liver recipients and antibody screening by flow cytometry
%D 2003
%@ 0041-1345
%U https://hdl.handle.net/20.500.14352/97963
%X Several authors have shown that anti-donor antibodies before liver transplantation are associated with decreased graft survival. The aim of this study was to investigate the relationship between anti-donor antibodies detected by the CDC technique or by FlowPRA, and acute or chronic rejection as well as graft survival. Furthermore, we sought to determine whether anti-donor antibodies, detected by the CDC technique, correlated with those discovered by cytometric screening. The acute rejection incidence among patients with complement-dependent cytotoxicity positive CDC cross-match was similar to that for patients with a negative cross-match. None of the patients with a positive cross-match developed chronic rejection. Allograft survival was significantly lower among recipients with a positive T-lymphocyte cross-match. Indeed, the majority of recipients with positive CDC cross-matches displayed graft failures before first postransplant year. The results of a positive FlowPRA determination were concordant with a positive CDC cross-match in 85.71% of cases. Our data demonstrate that pretransplant FlowPRA correlates with the final CDC cross-match results. This finding suggests that in the future prospective pretransplant antibody screening with FlowPRA or CDC techniques may be useful to identify high-risk recipients.
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