RT Journal Article
T1 Differential Metabolic and Transcriptional Responses ofGilthead Seabream (Sparus aurata) Administered with Cortisolor Cortisol-BSA
A1 Aedo, Jorge
A1 Aravena Canales, Daniela
A1 Ruiz Jarabo, Ignacio
A1 Oyarzún, Ricardo
A1 Molina, Alfredo
A1 Martínez Rodríguez, Gonzalo
A1 Valdés, Juan Antonio
A1 Mancera, Juan Miguel
AB Cortisol is the main glucocorticoid hormone promoting compensatory metabolic responses of stress in teleosts. This hormone acts through genomic and membrane-initiated actions to exert its functions inside the cell. Experimental approaches, using exogenous cortisol administration, confirm the role of this hormone during short (minutes to hours)- and long-term (days to weeks) responses to stress. The role of membrane-initiated cortisol signaling during long-term responses has been recently explored. In this study, Sparus aurata were intraperitoneally injected with coconut oil alone or coconut oil containing cortisol, cortisol-BSA, or BSA. After 3 days of treatment, plasma, liver, and skeletal muscle were extracted. Plasma cortisol, as well as metabolic indicators in the plasma and tissues collected, and metabolism-related gene expression, were measured. Our results showed that artificially increased plasma cortisol levels in S. aurata enhanced plasma glucose and triacylglycerols values as well as hepatic substrate energy mobilization. Additionally, cortisol stimulated hepatic carbohydrates metabolism, as seen by the increased expression of metabolism-related genes. All of these responses, observed in cortisol-administered fish, were not detected by replicating the same protocol and instead using cortisol-BSA, which exclusively induces membrane-initiated effects. Therefore, we suggest that after three days of cortisol administration, only genomic actions are involved in the metabolic responses in S. aurata.
PB MDPI
SN Electronic: 2076-2615
YR 2021
FD 2021-11-19
LK https://hdl.handle.net/20.500.14352/4919
UL https://hdl.handle.net/20.500.14352/4919
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
NO Fondo de Financiamiento de Centros de Investigación en Áreas Prioritarias (FONDAP)
NO Fondo Nacional de Desarrollo Científico y Tecnológico (FONDECYT)
DS Docta Complutense
RD 17 abr 2025