%0 Journal Article
%A Gamella Carballo, María
%A Ballesteros Redondo, María Isabel
%A Ruiz Valdepeñas Montiel, Víctor
%A Sánchiz, Africa
%A Cuadrado, Carmen
%A Pingarrón Carrazón, José Manuel
%A Linacero De La Fuente, M. Rosario
%A Campuzano Ruiz, Susana
%T Disposable amperometric biotool for peanut detection in processed foods by targeting a chloroplast DNA marker
%D 2024
%@ 0039-9140
%U https://hdl.handle.net/20.500.14352/112109
%X This work reports the development and application of a disposable amperometric sensor built on magnetic microcarriers coupled to an Express PCR strategy to amplify a specific DNA fragment of the chloroplast trnH-psbA. The procedure involves the selective capture of a 68-mer synthetic target DNA (or unmodified PCR products) through sandwich hybridization with RNA capture probe-modified streptavidin MBs and RNA signaling probes, labeled using antibodies specific to the heteroduplexes and secondary antibodies tagged with horseradish peroxidase. Amperometric measurements were performed on screen-printed electrodes using the H2O2/hydroquinone system. Achieving a LOD of 3 pM for the synthetic target, it was possible to detect 2.5 pg of peanut DNA and around 10 mg kg−1 of peanut in binary mixtures (defatted peanut flours prepared in spelt wheat). However, the detectability decreased between 10 and 1000 times in processed samples depending on the treatment. The Express PCR-bioplatform was applied to the detection of peanut traces in foodstuff.
%~