RT Journal Article
T1 Disposable amperometric biotool for peanut detection in processed foods by targeting a chloroplast DNA marker
A1 Gamella Carballo, María
A1 Ballesteros Redondo, María Isabel
A1 Ruiz Valdepeñas Montiel, Víctor
A1 Sánchiz, Africa
A1 Cuadrado, Carmen
A1 Pingarrón Carrazón, José Manuel
A1 Linacero De La Fuente, M. Rosario
A1 Campuzano Ruiz, Susana
AB This work reports the development and application of a disposable amperometric sensor built on magnetic microcarriers coupled to an Express PCR strategy to amplify a specific DNA fragment of the chloroplast trnH-psbA. The procedure involves the selective capture of a 68-mer synthetic target DNA (or unmodified PCR products) through sandwich hybridization with RNA capture probe-modified streptavidin MBs and RNA signaling probes, labeled using antibodies specific to the heteroduplexes and secondary antibodies tagged with horseradish peroxidase. Amperometric measurements were performed on screen-printed electrodes using the H2O2/hydroquinone system. Achieving a LOD of 3 pM for the synthetic target, it was possible to detect 2.5 pg of peanut DNA and around 10 mg kg−1 of peanut in binary mixtures (defatted peanut flours prepared in spelt wheat). However, the detectability decreased between 10 and 1000 times in processed samples depending on the treatment. The Express PCR-bioplatform was applied to the detection of peanut traces in foodstuff.
PB Elsevier
SN 0039-9140
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/112109
UL https://hdl.handle.net/20.500.14352/112109
LA eng
NO Gamella M, Ballesteros MI, Ruiz-Valdepeñas Montiel V, Sánchiz A, Cuadrado C, Pingarrón JM, Linacero R, Campuzano S. Disposable amperometric biotool for peanut detection in processed foods by targeting a chloroplast DNA marker. Talanta 2024;277:126350. https://doi.org/10.1016/j.talanta.2024.126350.
NO Acknowledgements:The financial support of Grants AGL2017-83082-R, PID2021-122942OB-I00 and PID2022-136351OB-I00 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe” is gratefully acknowledged.
NO European Commission
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 4 abr 2025