RT Journal Article
T1 Bi-enzymatic biosensor for on-site, fast and reliable electrochemical detection of relevant D-amino acids in bacterial samples
A1 Moreno Guzmán, María
A1 García-Carmona, Laura
A1 Molinero-Fernández, Águeda
A1 Cava, Felipe
A1 López Gil, Miguel Ángel
A1 Escarpa, Alberto
AB In this work, a bi-enzymatic biosensor allowed the total content of D-amino acids (DAAs) determination in highly relevant matrices involving bacteria. The strategy is based on the unique coimmobilization of D-amino acid oxidase (DAAO) and horseradish peroxidase (HRP) enzymes onto a multi-walled carbon nanotubes (MWCNTs) and gold nanoparticles (AuNPs) modified screen-printed electrode (SPCE). The greater amount of AuNPs deposited and hence the greater loading of both enzymes was observed when they were deposited after the activation of the carboxylated MWCNTs with EDC/Sulfo-NHS chemistry. These platforms provided a fast (300 s) and selective quantification of DAAs with excellent precision (RSD < 5%) and accuracy (Recoveries 100–104%) in bacterial samples. Collectively, the electrochemical bi-enzymatic biosensor become an universal, fast, sensitive and easy-to-use approach to determine total content of DAAs in complex matrices.
PB Elsevier
SN 0925-4005
YR 2017
FD 2017-04
LK https://hdl.handle.net/20.500.14352/93053
UL https://hdl.handle.net/20.500.14352/93053
LA eng
NO Moreno-Guzmán M, García-Carmona L, Molinero-Fernández Á, Cava F, López Gil MÁ, Escarpa A. Bi-enzymatic biosensor for on-site, fast and reliable electrochemical detection of relevant D-amino acids in bacterial samples. Sensors and Actuators B: Chemical 2017;242:95–101. https://doi.org/10.1016/j.snb.2016.11.037.
NO Ministerio de Economía y Competitividad (España)
NO NANOAVANSENS
NO Laboratory for Infection Medicine Sweden
NO Knut and Alice Wallenberg Foundation
NO Kempe foundation and the Swedish Research Council
NO Comunidad de Madrid
DS Docta Complutense
RD 18 abr 2025