RT Journal Article
T1 Antibacterial effect of 3D printed mesoporous bioactive glassscaffolds doped with metallic silver nanoparticles
A1 Sánchez Salcedo, Sandra
A1 García Fontecha, Ana
A1 González Jimenez, Adela
A1 Vallet Regí, María Dulce Nombre
AB The development of new biomaterials for bone tissue regeneration with high bioactivity abilities and antibacterial properties is being intensively investigated. We have synthesized nanocomposites formed by mesoporous bioactive glasses (MBGs) in the ternary SiO2, CaO and P2O5 system doped with metallic silver nanoparticles (AgNPs) that were homogenously embedded in the MBG matrices. Ag/MBG nanocomposites have been directly synthesized and silver species were spontaneously reduced to metallic AgNPs by high temperatures (700ºC) obtained of last MBG synthesis step. Three-dimensional silver-containing mesoporous bioactive glass scaffolds were fabricated showing uniformly interconnected ultrapores, macropores and mesopores. The manufacture method consisted of a combination of a single-step sol-gel route in the mesostructure directing agent (P123) presence and a biomacromolecular polymer such as (hydroxypropyl)methyl cellulose (HPMC) as the macrostructure template, followed by rapid prototyping (RP) technique. Biological properties of Ag/MBG nanocomposites were evaluated by MC3T3-E1 preosteoblastic cells culture tests and bacterial (E. coli and S. aureus) assays. The results showed that the MC3T3-E1 cells morphology was not affected while preosteoblastic proliferation decreased when the presence of silver increased. Antimicrobial assays indicated that bacterial growth inhibition and biofilm destruction were directly proportional to the increased presence of AgNPs in the MBG matrices. Furthermore, in vitro co-culture of MC3T3- E1 cells and S. aureus bacteria confirmed that AgNPs presence was necessary for antibacterial activity, and AgNPs slightly affected cell proliferation parameters. Therefore, 3D printed scaffolds with hierarchical pore structure and high antimicrobial capacity have potentialapplications in bone tissue regeneration.
PB Elsevier
SN 1742-7061
YR 2022
FD 2022-11-01
LK https://hdl.handle.net/20.500.14352/72657
UL https://hdl.handle.net/20.500.14352/72657
LA eng
NO CRUE-CSIC (Acuerdos Transformativos 2022)RESEARCH ID N-4501-2014  (Sandra Sánchez Salcedo)ORCID 0000-0002-1889-2057 (Sandra Sánchez Salcedo)RESEARCH ID B-1301-2015  (Ana García Fontecha)ORCID 0000-0002-8792-872X (Ana García Fontecha)RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)
NO Unión Europea. Horizonte 2020
NO Ministerio de Ciencia e Innovación (MICINN)
DS Docta Complutense
RD 2 may 2024