RT Journal Article
T1 A reagentless enzymatic fluorescent biosensor for glucose based on upconverting glasses, as excitation source, and chemically modified glucose oxidase
A1 Del Barrio Redondo, Melisa
A1 Cases, Rafael
A1 Cebolla, Vicente
A1 Hirsch, Thomas
A1 Marcos, Susana de
A1 Wilhelm, Stefan
A1 Galbán, Javier
AB Upon near-infrared excitation Tm3þ þYb3þ doped fluorohafnate glasses present upconversion properties and emit visible light. This property permits to use these glasses (UCG) as excitation sources for fluorescent optical biosensors. Taking this into account, in this work a fluorescent biosensor for glucose determination is designed and evaluated. The biosensor combines the UCG and the fluorescence of the enzyme glucose oxidase chemically modified with a fluorescein derivative (GOx-FS), whose intensity is modified during the enzymatic reaction with glucose. Optical parameters have been optimized and a mathematical model describing the behavior of the analytical signal is suggested. Working in FIA mode, the biosensor responds to glucose concentrations up to, at least, 15 mM with a limit of detection of 1.9 mM. The biosensor has a minimum lifetime of 9 days and has been applied to glucose determination in drinks. The applicability of the sensor was tested by glucose determination in two fruit juices
PB Elsevier
SN 0039-9140
YR 2016
FD 2016
LK https://hdl.handle.net/20.500.14352/92787
UL https://hdl.handle.net/20.500.14352/92787
LA eng
NO Del Barrio, Melisa, et al. «A Reagentless Enzymatic Fluorescent Biosensor for Glucose Based on Upconverting Glasses, as Excitation Source, and Chemically Modified Glucose Oxidase». Talanta, vol. 160, noviembre de 2016, pp. 586-91. https://doi.org/10.1016/j.talanta.2016.07.062.
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 20 abr 2025