RT Journal Article T1 Molecular identification of black-pigmented bacteria from subgingival samples of cats suffering from periodontal disease A1 Pérez Salcedo, L. A1 Laguna, E. A1 Sánchez, M. C. A1 Marín, M. J. A1 O`Connor, A. A1 González, I. A1 Sanz Alonso, Mariano A1 Herrera González, David AB Objectives: To characterise the black-pigmented bacterial species found in the subgingival samples of cats with periodontal disease using molecular-based microbiological techniques.Methods: Sixty-five subgingival samples obtained from 50 cats with periodontal disease were analysed by polymerase chain reaction amplified ribosomal DNA restriction analysis and cloning and sequencing of the 16S rRNA genes.Results: Among the 65 subgingival samples, eight phylogenetic profiles were obtained, of which the most prevalent species were: Porphyromonas gulae (40%), P. gingivalis/P. gulae (36 · 9%), P. gulae/Porphyromonas sp. UQD 406 (9 · 2%), Odoribacter denticanis (6 · 2%), P. gulae/Porphyromonas sp. UQD 348 (1 · 5%) and P. circumdentaria (1 · 5%). When compared with the species resulting from biochemical diagnosis, the identification of P. gulae was congruent in 70% of the cases, while colonies identified as P. intermedia-like corresponded in 80% of cases to P. gulae.Clinical significance: The use of molecular-based microbiological diagnostic techniques resulted in a predominance of Porphyromonas spp. in the subgingival plaque of cats suffering from periodontal disease. Further characterisation of these bacteria identified P. gulae, O. denticanis and P. circumdentaria. The more frequently detected phylogenetic profiles corresponded to P. gingivalis and P. gulae. PB Wiley SN 0022-4510 YR 2015 FD 2015-04 LK https://hdl.handle.net/20.500.14352/108009 UL https://hdl.handle.net/20.500.14352/108009 LA eng NO Pérez-Salcedo L, Laguna E, Sánchez MC, Marín MJ, O'Connor A, González I, Sanz M, Herrera D. Molecular identification of black-pigmented bacteria from subgingival samples of cats suffering from periodontal disease. J Small Anim Pract. 2015 Apr;56(4):270-5. doi: 10.1111/jsap.12319 DS Docta Complutense RD 4 abr 2025