RT Journal Article
T1 Comprehensive Characterization of a Porcine Model of The “Small-for-Flow” Syndrome
A1 Orue-Echebarria, Maitane
A1 Vaquero Martín, Francisco Javier
A1 Lisbona, Cristina
A1 Lozano, Pablo
A1 Steiner, Miguel
A1 Morales, Álvaro
A1 López Baena, José Ángel
A1 Laso, Juan
A1 Hernández, Inmaculada
A1 Olmedilla, Luis
A1 García Sabrido, José Luis
A1 Peligros Gómez, María Isabel
A1 Sola Vendrell, Emma
A1 Fernández Carballal, Carlos
A1 Vara Ameigeiras, Elena María
A1 Asencio Pascual, José Manuel
AB MethodsWe performed subtotal (90%) hepatectomies in 10 female MiniPigs using a simplified transection technique with a tourniquet. Blood tests, hepatic and systemic hemodynamics, and hepatic function and histology were assessed before (Bas), 15 min (t-15 min) and 24 h (t-24 h) after the operation. Some pigs underwent computed tomography (CT) scans for hepatic volumetry (n = 4) and intracranial pressure (ICP) monitoring (n = 3). Postoperative care was performed in an intensive care unit environment.ResultsAll hepatectomies were successfully performed, and hepatic volumetry confirmed liver remnant volumes of 9.2% [6.2–11.2]. The hepatectomy resulted in characteristic hepatic hemodynamic alterations, including portal hyperperfusion, relative decrease of hepatic arterial blood flow, and increased portal pressure (PP) and portal-systemic pressure gradient. The model reproduced major diagnostic features including the development of cholestasis, coagulopathy, encephalopathy with increased ICP, ascites, and renal failure, hyperdynamic circulation, and hyperlactatemia. Two animals (20%) died before t-24 h. Histological liver damage was observed at t-15 min and at t-24 h. The degree of histological damage at t-24 h correlated with intraoperative PP (r = 0.689, p = 0.028), hepatic arterial blood flow (r = 0.655, p = 0.040), and hepatic arterial pulsatility index (r = 0.724, p = 0.066). All animals with intraoperative PP > 20 mmHg presented liver damage at t-24 h.ConclusionThe present 90% hepatectomy porcine experimental model is a feasible and reproducible model for investigating the “Small-for-Flow” syndrome.
PB Elsevier
SN 1091-255X
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/100471
UL https://hdl.handle.net/20.500.14352/100471
LA eng
NO Maitane I. Orue-Echebarria, Javier Vaquero, Cristina J. Lisbona, Pablo Lozano, Miguel A. Steiner, Álvaro Morales, José Á. López-Baena, Juan Laso, Inmaculada Hernández, Luis Olmedilla, José L. García Sabrido, Isabel Peligros, Emma Sola, Carlos Carballal, Elena Vara, J.M. Asencio, Comprehensive Characterization of a Porcine Model of The “Small-for-Flow” Syndrome, Journal of Gastrointestinal Surgery, Volume 23, Issue 11, 2019, Pages 2174-2183,
NO Sociedad Española de Trasplante Hepático 
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 6 abr 2025