RT Journal Article
T1 Live Imaging Reveals Cerebellar Neural Stem Cell Dynamics and the Role of VNUT in Lineage Progression
T2 El registro continuo de imagen revela la dinámica de las células madre neurales cerebelosas y el papel de VNUT en la progresión del linaje
A1 Paniagua-Herranz, Lucía
A1 Menéndez-Méndez, Aida
A1 Gómez Villafuertes, María Rosa
A1 Olivos Ore, Luis Alcides
A1 Biscaia, Miguel
A1 Gualix Sánchez, Francisco Javier
A1 Pérez Sen, Raquel
A1 García Delicado, Esmerilda
A1 Rodríguez Artalejo, Antonio
A1 Miras Portugal, María Teresa
A1 Ortega De La O, Felipe
AB Little is known about the intrinsic specification of postnatal cerebellar neural stem cells (NSCs) and to what extent they depend on information from their local niche. Here, we have used an adapted cell preparation of isolated postnatal NSCs and live imaging to demonstrate that cerebellar progenitors maintain their neurogenic nature by displaying hallmarks of NSCs. Furthermore, by using this preparation, all the cell types produced postnatally in the cerebellum, in similar relative proportions to those observed in vivo, can be monitored. The fact that neurogenesis occurs in such organized manner in the absence of signals from the local environment, suggests that cerebellar lineage progression is to an important extent governed by cell-intrinsic or pre-programmed events. Finally, we took advantage of the absence of the niche to assay the influence of the vesicular nucleotide transporter inhibition, which dramatically reduced the number of NSCs in vitro by promoting their progression toward neurogenesis.
PB Cell Press
SN 2213-6711
YR 2020
FD 2020
LK https://hdl.handle.net/20.500.14352/96134
UL https://hdl.handle.net/20.500.14352/96134
LA eng
NO Comunidad de Madrid
NO Universidad Complutense de Madrid
NO Fundación Ramón Areces
NO Ministerio de Educación y Cultura (España)
NO Spanish Ministerio de Ciencia, Innovacio´n y Universidades
DS Docta Complutense
RD 8 abr 2025