RT Journal Article
T1 Leptin but not neuropeptide Y up-regulated glucagon-like peptide 1 receptor expression in GT1-7 cells and rat hypothalamic slices
A1 Sanz Carmen, 
A1 Vázquez Patricia, 
A1 Navas M. Angeles, 
A1 Alvarez Elvira, 
A1 Blázquez Enrique, 
A2 Elsevier BV, 
AB In an attempt to gain better insight into the central effects of glucagon-like peptide (GLP-1), we studied the action of glucose and of regulatory peptides on the expression of its receptor (GLP-1R) in hypothalamic GT1-7 cells and in ventromedial (VMH) and lateral (LH) rat hypothalamus slices. The promoter activity of GLP-1R in transfected GT1-7 cells increased with leptin, whereas neuropeptide Y (NPY) did not modify it. Interestingly, when cells were incubated with both NPY and leptin, NPY blocked the stimulating effect of leptin. The effects of leptin and NPY were also confirmed at messenger RNA levels. In hypothalamic slices, GLP-1R messenger RNA levels increased at higher glucose concentrations in the VMH. In addition, leptin exerted a stimulating effect; and NPY did not modify receptor expression. By contrast, in the LH, the opposite effects were found for those parameters, except at 20 mmol/L glucose. These findings suggest that the stimulating effect of leptin on GLP-1R expression, with no changes in NPY-induced activity, could enhance the anorexic actions generated through this receptor. In addition, the different responses of the VMH and LH may be related to specific functions of these structures, as already known in vivo, highlighting the interest of hypothalamic slices for this kind of study.
PB Elsevier
SN 0026-0495
YR 2007
FD 2007-08-03
LK https://hdl.handle.net/20.500.14352/96215
UL https://hdl.handle.net/20.500.14352/96215
LA eng
NO Sanz C, Vázquez P, Navas MA, Alvarez E, Blázquez E. Leptin but not neuropeptide Y up-regulated glucagon-like peptide 1 receptor expression in GT1-7 cells and rat hypothalamic slices. Metabolism. 2008;57(1):40-48. doi:10.1016/j.metabol.2007.08.005
DS Docta Complutense
RD 8 abr 2025