RT Journal Article
T1 Intracellular Mechanical Drugs Induce Cell‐Cycle Altering and Cell Death
A1 Arjona, María Isabel
A1 Vázquez Pérez, Patricia
A1 Plaza, José A.
AB Current advances in materials science have demonstrated that extracellular mechanical cues can define cell function and cell fate. However, a fundamental understanding of the manner in which intracellular mechanical cues affect cell mechanics remains elusive. How intracellular mechanical hindrance, reinforcement, and supports interfere with the cell cycle and promote cell death is described here. Reproducible devices with highly controlled size, shape, and with a broad range of stiffness are internalized in HeLa cells. Once inside, they induce characteristic cell-cycle deviations and promote cell death. Device shape and stiffness are the dominant determinants of mechanical impairment. Device structural support to the cell membrane and centering during mitosis maximize their effects, preventing spindle centering, and correct chromosome alignment. Nanodevices reveal that the spindle generates forces larger than 114 nN which overcomes intracellular confinement by relocating the device to a less damaging position. By using intracellular mechanical drugs, this work provides a foundation to defining the role of intracellular constraints on cell function and fate, with relevance to fundamental cell mechanics and nanomedicine.
PB Wiley
SN 0935-9648
SN 1521-4095
YR 2022
FD 2022-03-21
LK https://hdl.handle.net/20.500.14352/112380
UL https://hdl.handle.net/20.500.14352/112380
LA eng
NO Arjona, María Isabel, et al. «Intracellular Mechanical Drugs Induce Cell‐Cycle Altering and Cell Death». Advanced Materials, vol. 34, n.o 17, abril de 2022, p. 2109581. DOI.org (Crossref), https://doi.org/10.1002/adma.202109581.
NO Fondos FEDER
NO European Commission
NO Ministerio de Economía, Industria y Competitividad (España)
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 8 abr 2025