RT Journal Article
T1 Wide diversity of parasites in Bombus terrestris (Linnaeus, 1758) revealed by a high-throughput sequencing approach
A1 Bartolomé, Carolina
A1 Jabal-Uriel, Clara
A1 Buendía-Abad, María
A1 Benito León, María
A1 Ornosa Gallego, Concepción
A1 Rúa, Pilar de la
A1 Martín-Hernández, Raquel
A1 Higes, Mariano
A1 Maside, Xulio
AB Assessing the extent of parasite diversity requires the application of appropriate molecular tools, especially given the growing evidence of multiple parasite co-occurrence. Here, we compared the performance of a next-generation sequencing technology (Ion PGM ™ System) in 12 Bombus terrestris specimens that were PCR-identified as positive for trypanosomatids (Leishmaniinae) in a previous study. These bumblebees were also screened for the occurrence of Nosematidae and Neogregarinorida parasites using both classical protocols (either specific PCR amplification or amplification with broadrange primers plus Sanger sequencing) and Ion PGM sequencing. The latter revealed higher parasite diversity within individuals, especially among Leishmaniinae (which were present as a combination of Lotmaria passim, Crithidia mellificae and Crithidia bombi), and the occurrence of taxa never reported in these hosts: Crithidia acanthocephali and a novel neogregarinorida species. Furthermore, the complementary results produced by the different sets of primers highlighted the convenience of using multiple markers to minimize the chance of some target organisms going unnoticed. Altogether, the deep sequencing methodology offered a more comprehensive way to investigate parasite diversity than the usual identification methods and provided new insights whose importance for bumblebee health should be further analysed.
PB Wiley
SN 1462-2912; Electronic: 1462-2920
YR 2020
FD 2020-11-22
LK https://hdl.handle.net/20.500.14352/8002
UL https://hdl.handle.net/20.500.14352/8002
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
NO Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)/Fondo Europeo de Desarrollo Regional (FEDER)
NO Fundación Seneca-Agencia de Ciencia y Tecnología de la Región de Murcia
DS Docta Complutense
RD 15 may 2024