RT Journal Article
T1 In vitro biofilm formation by Streptococcus pneumoniae as a predictor of post‐vaccination emerging serotypes colonizing the human nasopharynx
A1 Domenech Lucas, Miriam
A1 Araújo‐Bazán, Lidia
A1 García, Ernesto
A1 Moscoso, Miriam
AB The increasing use of the 7-valent pneumococcal conjugate vaccine has been accompanied by the rise of non-vaccine serotypes colonizing the human nasopharynx. The vast majority of infections are caused by microorganisms that grow in biofilms. It has recently been shown that the formation of Streptococcus pneumoniae biofilms in vivo and in vitro is hindered by the presence of capsular polysaccharide. The biofilm-forming capacity of pneumococcal clinical isolates with different types of capsular polysaccharide and various isogenic transformants was examined. Strains of serotypes 19A and 19F, but not 19B and 19C, formed ≥ 80% of the quantity of biofilm associated with a non-encapsulated control strain. Strains of serogroup 6 also showed significant biofilm-forming capacity. The capsules of serotypes 19A and 19F, and serogroup 6 contain the disaccharides α-D-Glcp-(1→2)-α-L-Rhap-(1→ and α-D-Glcp-(1→3)-α-L-Rhap-(1→. Serotype 18A and serotypes 18B/18C have very similar capsular disaccharides: α-D-GlcpNAc-(1→3)-β-L-Rhap-(1→ and α-D-Glcp-(1→3)-β-L-Rhap-(1→ respectively. However, the strains of serogroup 18 showed impaired biofilm formation. These results indicate that the chemical composition/structure of the capsular polysaccharide is crucial to the biofilm-forming capacity of pneumococcal serotypes. Testing of the in vitro biofilm-forming ability of isogenic transformants expressing different capsular polysaccharides may help predict the emergence of colonizing, non-vaccine serotypes.
PB Wiley
SN 1462-2912
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/97729
UL https://hdl.handle.net/20.500.14352/97729
LA eng
NO Domenech, Mirian, et al. «In Vitro Biofilm Formation by S Treptococcus Pneumoniae as a Predictor of Post‐vaccination Emerging Serotypes Colonizing the Human Nasopharynx». Environmental Microbiology, vol. 16, n.o 4, abril de 2014, pp. 1193-201. https://doi.org/10.1111/1462-2920.12370.
NO Ministerio de Economía y Competitividad (España)
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 2 oct 2024