RT Journal Article
T1 Retinal Ganglion Cell Loss and Microglial Activation in a SOD1G93A Mouse Model of Amyotrophic Lateral Sclerosis
A1 Rojas Lozano, María Del Pilar
A1 Ramírez Sebastián, Ana Isabel
A1 Cadena Santoyo, Manuel
A1 Fernández Arrabal, José Antonio
A1 García Martín, Elena Salobrar
A1 López Cuenca, Inés
A1 Santos García, Irene
A1 Lago Femia, Eva De
A1 Urcelay Segura, José Luis
A1 Ramírez Sebastián, José Manuel
A1 Hoz Montañana, María Rosa De
A1 Salazar Corral, Juan José
AB The neurodegenerative disease amyotrophic lateral sclerosis (ALS) affects the spinal cord, brain stem, and cerebral cortex. In this pathology, both neurons and glial cells are affected. However, few studies have analyzed retinal microglia in ALS models. In this study, we quantified the signs of microglial activation and the number of retinal ganglion cells (RGCs) in an SOD1G93A transgenic mouse model at 120 days (advanced stage of the disease) in retinal whole-mounts. For SOD1G93A animals (compared to the wild-type), we found, in microglial cells, (i) a significant increase in the area occupied by each microglial cell in the total area of the retina; (ii) a significant increase in the arbor area in the outer plexiform layer (OPL) inferior sector; (iii) the presence of cells with retracted processes; (iv) areas of cell groupings in some sectors; (v) no significant increase in the number of microglial cells; (vi) the expression of IFN-γ and IL-1β; and (vii) the non-expression of IL-10 and arginase-I. For the RGCs, we found a decrease in their number. In conclusion, in the SOD1G93A model (at 120 days), retinal microglial activation occurred, taking a pro-inflammatory phenotype M1, which affected the OPL and inner retinal layers and could be related to RGC loss
PB MDPI
SN 1661-6596
YR 2021
FD 2021-02-07
LK https://hdl.handle.net/20.500.14352/7792
UL https://hdl.handle.net/20.500.14352/7792
LA eng
NO Rojas Lozano, P., Ramírez Sebastián, A. I., Cadena Santoyo, M. et al. «Retinal Ganglion Cell Loss and Microglial Activation in a SOD1G93A Mouse Model of Amyotrophic Lateral Sclerosis». International Journal of Molecular Sciences, vol. 22, n.o 4, febrero de 2021, p. 1663. DOI.org (Crossref), https://doi.org/10.3390/ijms22041663.
NO This article belongs to the Special Issue Retinal Neurodegenerative Diseases: Molecular Targets Driving Neuroinflammation and Neuroprotection.
NO Ministerio de Economía, Comercio y Empresa (España)
NO Instituto de Salud Carlos III
NO Fondo Europeo de Desarrollo Regional
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Comunidad de Madrid
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 10 abr 2025