RT Journal Article
T1 Cecal Reduction of Brachyspira and Lesion Severity in Laying Hens Supplemented with Fermented Defatted ‘Alperujo’
A1 Rebollada Merino, Agustín Miguel
A1 Ugarte Ruiz, María
A1 Gómez-Buendía, Alberto
A1 Bárcena Asensio, María Carmen
A1 García Benzaquén, Nerea
A1 Domínguez Rodríguez, Lucas José
A1 Rodríguez Bertos, Antonio Manuel
AB Antimicrobial resistance demands the development of therapeutic alternatives such as prebiotics, probiotics, and nutraceuticals. The aim of this study was to assess the antimicrobial proprieties of the nutraceutical fermented defatted “alperujo”, derived from olive oil production, in a laying hen farm (n = 122,250) endemic with avian intestinal spirochetosis (Brachyspira spp.). Part of the batch (n = 1440) was divided into six groups of 240 hens each that included 80 or 108-week-old laying hens, supplemented with 0%, 2%, or 6% fermented defatted ‘alperujo’ for a month. At the end of the experiment, eight hens from each group were autopsied and cecal content was subjected to (i) Brachyspira culture and species identification by PCRs, and (ii) direct DNA extraction and Brachyspira qPCR. Furthermore, the ceca were processed for histopathology. Microbiological isolation revealed B. pilosicoli and B. hyodysenteriae co-infection in all groups. The 80-week-old hen group 2% supplemented showed a reduction in the cecal Brachyspira content (qPCR) compared with non-supplemented hens. Cecal histopathology showed a diffuse mild infiltration of lymphocytes, plasma cells, and heterophils; and hyperplasia of the gut-associated lymphoid tissue hyperplasia which decreased in severity in 80-week-old supplemented hens. The reduction in Brachyspira colonization and the severity of the lesions observed in supplemented hens highlights a potential protective function against avian intestinal spirochetosis.
PB MDPI
SN 2311-5637
YR 2022
FD 2022
LK https://hdl.handle.net/20.500.14352/93232
UL https://hdl.handle.net/20.500.14352/93232
LA eng
NO Rebollada-Merino, A.; Ugarte-Ruiz, M.; Gómez-Buendía, A.; Bárcena, C.; García, N.; Domínguez, L.; Rodríguez-Bertos, A. Cecal Reduction of Brachyspira and Lesion Severity in Laying Hens Supplemented with Fermented Defatted ‘Alperujo’. Fermentation 2022, 8, 550. https://doi.org/ 10.3390/fermentation8100550
NO Ministerios de Economía, Industria y Competitividad (España)
NO Comunidad de Madrid
NO Ministerio de Ciencia e Innovación (España)
NO Ministerio de Universidades (España)
DS Docta Complutense
RD 9 abr 2025