RT Journal Article
T1 Effects of a mesoporous bioactive glass on osteoblasts, osteoclasts and macrophages
A1 Gómez Cerezo, María Natividad
A1 Casarrubios Molina, Laura
A1 Morales, I.
A1 Feito Castellano, María José
A1 Vallet Regí, María Dulce Nombre
A1 Arcos Navarrete, Daniel
A1 Portolés Pérez, María Teresa
AB A mesoporous bioactive glass (MBG) of molar composition 75SiO2-20CaO-5P2O5 (MBG-75S) has been synthetized as a potential bioceramic for bone regeneration purposes. X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), nitrogen adsorption studies and transmission electron microscopy (TEM) demonstrated that MBG-75S possess a highly ordered mesoporous structure with high surface area and porosity, which would explain the high ionic exchange rate (mainly calcium and silicon soluble species) with the surrounded media. MBG-75S showed high biocompatibility in contact with Saos-2 osteoblast-like cells. Concentrations up to 1 mg/ml did not lead to significant alterations on either morphology or cell cycle. Regarding the effects on osteoclasts, MBG-75S allowed the differentiation of RAW264.7 macrophages into osteoclast-like cells but exhibiting a decreased resorptive activity. These results point out that MBG-75S does not inhibit osteoclastogenesis but reduces the osteoclast bone-resorbing capability. Finally, in vitro studies focused on the innate immune response, evidenced that MBG-75S allows the proliferation of macrophages without inducing their polarization towards the M1 pro-inflammatory phenotype. This in vitro behavior is indicative that MBG-75S would just induce the required innate immune response without further inflammatory complications under in vivo conditions. The overall behavior respect to osteoblasts, osteoclasts and macrophages, makes this MBG a very interesting candidate for bone grafting applications in osteoporotic patients.
PB Elsevier
SN 0021-9797
YR 2018
FD 2018-10-15
LK https://hdl.handle.net/20.500.14352/12150
UL https://hdl.handle.net/20.500.14352/12150
LA eng
NO RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)RESEARCHER ID L-6167-2014 (Daniel Arcos Navarrete)ORCID 0000-0002-5367-7272 (Daniel Arcos Navarrete)RESEARCHER ID U-1678-2017 (María Teresa Portolés Pérez)ORCID 0000-0002-9681-0184 (María Teresa Portolés Pérez)
NO Unión Europea. H2020
NO Ministerio de Economía y Competitividad (MINECO)
NO Comunidad de Madrid
NO Fondos FEDER
DS Docta Complutense
RD 9 abr 2025