RT Journal Article
T1 Construction of a Fab Library Merging Chains from Semisynthetic and Immune Origin, Suitable for Developing New Tools for Gluten Immunodetection in Food
A1 García Calvo, Eduardo Rafael
A1 García García, Aina
A1 Rodríguez, Santiago
A1 Farrais, Sergio
A1 Martín, Rosario
A1 García, Teresa
AB The observed increase in the prevalence of gluten-related disorders has prompted the development of novel immunological systems for gluten detection in foodstuff. The innovation on these methods relies on the generation of new antibodies, which might alternatively be obtained by molecular evolution methods such as phage display. This work presents a novel approach for the generation of a Fab library by merging semi-synthetic heavy chains built-up from a pre-existent recombinant antibody fragment (dAb8E) with an immune light chain set derived from celiac donors. From the initial phage population (107 candidates) and after three rounds of selection and amplification, four different clones were isolated for further characterization. The phage Fab8E-4 presented the best features to be applied in an indirect ELISA for the detection of gluten in foods, resulting in improved specificity and sensitivity.
PB MPDI
SN 2304-8158
YR 2022
FD 2022-12-28
LK https://hdl.handle.net/20.500.14352/73196
UL https://hdl.handle.net/20.500.14352/73196
LA eng
NO Ministerio de Ciencia e Innovación
NO Comunidad de Madrid /FSE
DS Docta Complutense
RD 14 dic 2025