RT Journal Article
T1 Competitive ligand binding kinetics to linear polymers
A1 García Villaluenga, Juan Pedro
A1 Brunete, David
A1 Cao García, Francisco Javier
AB Different types of ligands compete in binding to polymers with different consequences for the physical and chemical properties of the resulting complex. Here, we derive a general kinetic model for the competitive binding kinetics of different types of ligands to a linear polymer, using the McGhee and von Hippel detailed binding-site counting procedure. The derived model allows the description of the competitive binding process in terms of the size of the ligand, binding, and release rates, and cooperativity parameters. We illustrate the implications of the general theory showing the equations for the competitive binding of two ligands. The size of the ligand, given by the number of monomers occluded, is shown to have a great impact on competitive binding. Ligands requiring a large available gap for binding are strongly inhibited by smaller ligands. Ligand size then has a leading role compared to binding affinity or cooperativity. For ligands that can bind in different modes (i.e., different number of monomers), this implies that they are more effective in covering or passivating the polymer in lower modes, if the different modes have similar binding energies.
PB American Physical Society
SN 2470-0045
YR 2023
FD 2023-02
LK https://hdl.handle.net/20.500.14352/104351
UL https://hdl.handle.net/20.500.14352/104351
LA eng
NO Villaluenga, J. P., Brunete, D., & Cao-García, F. J. (2023). Competitive ligand binding kinetics to linear polymers. Physical Review E, 107(2), 024401.
NO Ministerio de Economía y Competitividad (España)
NO European Commission
DS Docta Complutense
RD 6 abr 2025