%0 Journal Article
%A Ahijado Guzmán, Rubén
%A Prasad, Janak
%A Rosman, Christina
%A Henkel, Andreas
%A Tome, Lydia
%A Schneider, Dirk
%A Rivas, Germán
%A Sönnichsen, Carsten
%T Plasmonic nanosensors for simultaneous quantification of multiple protein–protein binding affinities
%D 2014
%@ 1556-276X
%U https://hdl.handle.net/20.500.14352/92112
%X Most of current techniques used for the quantification of protein–protein interactions require the analysis of one pair of binding partners at a time. Herein we present a label-free, simple, fast, and cost-effective route to characterize binding affinities between multiple macromolecular partners simultaneously, using optical dark-field spectroscopy and individual protein-functionalized gold nanorods as sensing elements. Our NanoSPR method could easily become a simple and standard tool in biological, biochemical, and medical laboratories.
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