RT Journal Article
T1 Plasmonic nanosensors for simultaneous quantification of multiple protein–protein binding affinities
A1 Ahijado Guzmán, Rubén
A1 Prasad, Janak
A1 Rosman, Christina
A1 Henkel, Andreas
A1 Tome, Lydia
A1 Schneider, Dirk
A1 Rivas, Germán
A1 Sönnichsen, Carsten
AB Most of current techniques used for the quantification of protein–protein interactions require the analysis of one pair of binding partners at a time. Herein we present a label-free, simple, fast, and cost-effective route to characterize binding affinities between multiple macromolecular partners simultaneously, using optical dark-field spectroscopy and individual protein-functionalized gold nanorods as sensing elements. Our NanoSPR method could easily become a simple and standard tool in biological, biochemical, and medical laboratories.
PB American Chemical Society
SN 1556-276X
YR 2014
FD 2014
LK https://hdl.handle.net/20.500.14352/92112
UL https://hdl.handle.net/20.500.14352/92112
LA eng
NO Ahijado-Guzmán, Rubén, et al. «Plasmonic Nanosensors for Simultaneous Quantification of Multiple Protein–Protein Binding Affinities». Nano Letters, vol. 14, n.o 10, octubre de 2014, pp. 5528-32. https://doi.org/10.1021/nl501865p.
NO European Commission
NO Agencia Estatal de Investigación (España)
NO Johannes Gutenberg University Mainz (Alemania)
DS Docta Complutense
RD 6 abr 2025