RT Journal Article
T1 Molecular Characterization of the Gene Encoding the DNA  Gyrase A Subunit of Streptococcus pneumoniae
A1 Balas, Delia
A1 Fernández Moreira, Esteban
A1 Campa, Adela G. de la
AB The gene encoding the DNA gyrase A subunit of Streptococcus pneumoniae was cloned and sequenced. The gyrA gene codes for a protein of 822 amino acids homologous to the gyrase A subunit of eubacteria. Translation of the gene in an Escherichia coli expression system revealed a 92-kDa polypeptide. A sequence-directed DNA curvature was identi®ed in the promoter region of gyrA. The bend center was mapped and located between the 35 and 10regions of the promoter. Primer extension analysis showed that gyrA transcription initiates 6 bp downstream of an extended 10 promoter. The possible implications of the bent DNA region as a regulatory element in the transcription of gyrA are discussed.
PB American Society for Microbiology
SN 0021-9193
YR 1998
FD 1998-06-01
LK https://hdl.handle.net/20.500.14352/124943
UL https://hdl.handle.net/20.500.14352/124943
LA eng
NO Balas D, Fernández-Moreira E, De La Campa AG. 1998. Molecular Characterization of the Gene Encoding the DNA Gyrase A Subunit of Streptococcus pneumoniae. J Bacteriol 180:. https://doi.org/10.1128/jb.180.11.2854-2861.1998
NO Ministerio de Sanidad (España)
DS Docta Complutense
RD 22 mar 2026