RT Journal Article
T1 Carob fruit extract-enriched meat improves pancreatic beta-cell dysfunction, hepatic insulin signaling and lipogenesis in late-stage type 2 diabetes mellitus model
A1 Macho González, Adrián
A1 López-Oliva Muñoz, María Elvira
A1 Merino Martín, José Joaquín
A1 García Fernández, Rosa Ana
A1 Garcimartín Álvarez, Alba
A1 Bastida Codina, Sara
A1 Redondo Castillejo, Rocío
A1 Sánchez Muniz, Francisco José
A1 Benedí González, Juana María
AB The inclusion of functional bioactive compounds of dietary fiber in meat products has been demonstrated to exert a significant impact on human health. Carob fruit extract (CFE) is a dietary fiber rich in proanthocyanidins with known antioxidant, hypolipidemic and hypoglycemic effects. Consumption of CFE-enriched meat (CFE-RM) may provide interesting benefits in late-stage type 2 diabetes mellitus (T2DM). To explore the antidiabetic mechanisms of CFE-RM, we used a model of late-stage T2DM in Wistar rats fed a high-saturated-fat/high-cholesterol diet (Chol-diet) and injected streptozotocin plus nicotinamide (D group). The effects of CFE-RM were tested by incorporating it into the diet as preventive strategy (ED group) or curative treatment (DE group). CFE-RM had a positive effect on glycemia, enhancing hepatic insulin sensitivity and improving pancreatic β-cell regeneration in both ED and DE groups. Western blotting and immunohistochemistry suggested that CFE-RM increased levels of insulin receptor β and phosphatidylinositol-3-kinase, as well as the downstream target phospho-Akt (at Ser473). CFE-RM also up-regulated glucose transporter 2, which improves the insulin-mediated glucose uptake by the liver, and promoted phosphorylation of glycogen synthesis kinase-3βprotein (at ser9), consequently increasing the hepatic glycogen content. In addition, CFE-RM decreased fatty liver by suppressing de novo lipogenesis activation due to down-regulation of liver X receptor-α/β, sterol regulatory element binding protein-1c and carbohydrate-response element-binding protein transcription factors. Our findings suggest that the consumption of CFE-RM included in the diet as a functional food should be considered as a suitable nutritional strategy to prevent or manage late-stage T2DM.
PB Elsevier
SN 0955-2863
YR 2020
FD 2020-10
LK https://hdl.handle.net/20.500.14352/92352
UL https://hdl.handle.net/20.500.14352/92352
LA eng
NO Macho-González A, López-Oliva ME, Merino JJ, García-Fernández RA, Garcimartín A, Redondo-Castillejo R, et al. Carob fruit extract-enriched meat improves pancreatic beta-cell dysfunction, hepatic insulin signaling and lipogenesis in late-stage type 2 diabetes mellitus model. The Journal of Nutritional Biochemistry 2020;84:108461. https://doi.org/10.1016/j.jnutbio.2020.108461.
NO Ministerio de Educación, Cultura y Deporte (España)
NO Banco Santander
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 7 abr 2025