RT Journal Article
T1 Comparative genomics and evolutionary analysis of Lactococcus garvieae isolated from human endocarditis
A1 Francés-Cuesta, Carlos
A1 Ansari, Iván
A1 Fernández-Garayzábal Fernández, José Francisco
A1 Gibello Prieto, Alicia
A1 González-Candelas, Fernando
AB Lactococcus garvieae is a well-known pathogen of fish, but is rarely involved in infections in humans and other mammals. In humans, the main clinical manifestation of L. garvieae infections is endocarditis usually related to the ingestion of contaminated food, such as undercooked fish and shellfish. This study presents the first complete genomic sequence of a clinical L. garvieae strain isolated from a patient with endocarditis and its comparative analysis with other genomes. This human isolate contains a circular chromosome of 2 099 060 bp and one plasmid of 50 557 bp. In comparison with other fully sequenced L. garvieae strains, the chromosomal DNA of L. garvieae Lg-Granada carries a low proportion of insertion sequence elements and a higher number of putative prophages. Our results show that, in general, L. garvieae is a highly recombinogenic species with an open pangenome in which almost 30 % of its genome has undergone horizontal transfers. Within the genus Lactococcus, L. lactis is the main donor of genetic components to L. garvieae but, taking Lg-Granada as a representative, this bacterium tends to importmore genes from Bacilli taxa than from other Lactococcus species.
PB Microbiology Society
SN 2057-5858
YR 2022
FD 2022
LK https://hdl.handle.net/20.500.14352/94388
UL https://hdl.handle.net/20.500.14352/94388
LA eng
NO Francés-Cuesta, C., Ansari, I., Fernández-Garayzábal, J. F., Gibello, A., & González-Candelas, F. (2022). Comparative genomics and evolutionary analysis of Lactococcus garvieae isolated from human endocarditis. Microbial genomics, 8(2), 000771
NO This is an open-access article distributed under the terms of the Creative Commons Attribution NonCommercial License. This article was made open access via a Publish and Read agreement between the Microbiology Society and the corresponding author’s institution.This work was supported by the Spanish Ministry of Science, Innovation, and Universities [RTI2018-098530-B-I00 and BFU2017-89594-R]. C-F.C. was supported by the Spanish Ministry of Science, Innovation, and Universities [BES-2015-074204].
NO Ministerio de Ciencia, Innovación y Universidades (España)
DS Docta Complutense
RD 20 abr 2025