RT Journal Article
T1 Fluctuation analysis to select for Samarium bio-uptaking microalgae clones the repurposing of a classical evolution experiment
A1 Martínez-Alesón García, Paloma
A1 García Balboa, María Del Camino
A1 Romero-López, Julia
A1 López Rodas, Victoria
A1 Costas Costas, Eduardo
A1 Baselga Cervera, Beatriz
AB Rare Earth Elements (REE) increasing demand prompts the research of biotechnological approaches to exploit secondary resources. We made use of the adapted Fluctuation analyses experiment to obtain Chlamydomonas reinhardtii ChlA strains resistant to Samarium (Sm) as the reference REE. The starting hypothesis was that adaptation to metal-containing media leads to an enhanced metal uptake. ChlA was able to adapt to 1.33·10−4 Sm M and pH~3 by pre-existing genetic variability, allowing the evolutionary rescue of 13 of the 99 populations studied. The rescuing resistant genotypes presented a mutation rate of 8.65·10−7 resistant cells per division. The resulting resistant population contradicted the expected fitness cost associated with the adaptation to Sm, selection resulted in larger and faster-growing resistant cells. Among the three isolated strains studied for Sm uptake, only one presented uplifted performance compared to the control population (46.64 μg Sm gˉ¹ of wet biomass and 3.26·10−7 ng Sm per cell, mainly bioaccumulated within the cells). The selection of microalgae strains with improved tolerance to REEs by this methodology could be a promising solution for REES sequestration. However, increased tolerance can be independent or have negative effects on uptake performance and cellular features studied are not directly correlated with the metal uptake.
PB Elsevier
SN 0147-6513
YR 2021
FD 2021-06-01
LK https://hdl.handle.net/20.500.14352/115885
UL https://hdl.handle.net/20.500.14352/115885
LA eng
NO Martínez-Alesón García, Paloma; García Balboa, Camino; Romero López, Julia; López Rodas, Victoria; Costas Costas, Eduardo; Baselga Cervera, Beatriz. Fluctuation analysis to select for Samarium bio-uptaking microalgae clones the repurposing of a classical evolution experiment. Ecotoxicology and Environmental Safety. 215 - 112134, Elsevier, 01/06/2021.
NO Author contributions: Paloma Martínez-Alesón García: Investigation, Formal analysis, Writing - Original Draft, Writing - Review & Editing, Camino García-Balboa: Definition, Methodology, Resources, Investigation, Writing - Review & Editing, Julia Romero-López: Writing - Review & Editing, Victoria López-Rodas: Definition, Supervision, Funding acquisition, Eduardo Costas: Definition, Supervision, Funding acquisition, Beatriz Baselga-Cervera: Definition, Conceptualization, Formal analysis, Visualization, Writing - Original Draft, Writing - Review & Editing.
NO Fundación Alsonso Martín Escudero
DS Docta Complutense
RD 25 abr 2025