RT Journal Article
T1 Anti-inflammatory effects of TNF-α and ASK1 inhibitory compounds isolated from Schkuhria pinnata used for the treatment of dermatitis
A1 Apaza Ticona, Luis Nestor
A1 Hervás Povo, Belén
A1 Sánchez Sánchez-Corral, Javier
A1 Rumbero Sánchez, Ángel
AB Ethno-pharmacological relevance: The Andean Schkuhria pinnata species commonly known as ‘Canchalagua’ is used as an infusion in Andean countries to treat various anti-inflammatory and skin-related pathologies.Aim of the study: This study determined the anti-inflammatory activity of the aqueous extract from Schkuhria pinnata, identified compounds with high biological activity and performed a structure-activity relationship analysis to determine their binding mechanism.Materials and methods: A bio-guided isolation of the active compounds of Schkuhria pinnata was carried out by selecting the most active sub-extracts and fractions to test their anti-inflammatory activity against the ASK1 and TNF-α cytokines.Results: Three compounds were obtained, and their structures were elucidated by nuclear magnetic resonance. The compounds were (3R,4R)-4-(3,4-dimethoxybenzyl)-3-(4-hydroxy-3-methoxybenzyl) dihydrofuran-2(3H)-one (1), N-[2,3-dihydro-1,3-dimethyl-6-[(2R)-2-methyl-1-piperazinyl]-2-oxo-1H-benzimidazol-5-yl]-2-methoxybenzamide (2), and N-hydroxy-1-cyclopentene-1-carboxamide (3). Regarding their anti-inflammatory activity, the three compounds inhibited the TNF-α and ASK1 cytokines, however, compound 2 was the most active, with an IC50 of 19.08 and 8.94 nM, respectively.Conclusion: The anti-inflammatory activity of the aqueous extract of Schkuhria pinnata was evaluated, followed by the isolation of three compounds and the study of their pharmacological activity. The three compounds have been shown as promising treatment against dermatitis, confirming at the same time their traditional use.
PB Elsevier
SN 0378-8741
YR 2023
FD 2023-08-19
LK https://hdl.handle.net/20.500.14352/108884
UL https://hdl.handle.net/20.500.14352/108884
LA eng
NO Luis Apaza Ticona, Belén Hervás Povo, Javier Sánchez Sánchez-Corral, Ángel Rumbero Sánchez, Anti-inflammatory effects of TNF-α and ASK1 inhibitory compounds isolated from Schkuhria pinnata used for the treatment of dermatitis, Journal of Ethnopharmacology, Volume 318, Part B, 2024, 117051, https://doi.org/10.1016/j.jep.2023.117051.
NO 2023 Acuerdos transformativos CRUE
NO Universidad Autónoma de Madrid
DS Docta Complutense
RD 16 abr 2025