%0 Journal Article
%A Andreu-Ballester, Juan Carlos
%A Hurtado-Marcos, Carolina
%A García-Ballesteros, Carlos
%A Pérez-Griera, Jaime
%A Ollero, Dolores
%A Jiménez, Ana
%A Gil-Borrás, Rafael
%A Llombart-Cussac, Antonio
%A López-Chuliá, Francisca
%A Izquierdo Ruiz, Fernando
%A Cuéllar Del Hoyo, María Del Carmen
%T Decreased gene expression of interleukin 2 receptor subunit γ (CD132) in tissues of patients with Crohn’s disease
%D 2025
%@ 1007-9327
%U https://hdl.handle.net/20.500.14352/129744
%X Background: A deficiency of γδ T cells has been described in Crohn's disease (CD).Aim: To analyze the gene expression of interleukin 7 (IL-7) and its receptors in the tissues of patients with CD.Methods: We studied the peripheral blood of 80 patients with CD, comparing them with a group of 80 healthy subjects. The number and apoptosis of αβ and γδ T cells in peripheral blood and the proportion of αβ and γδ T cells in the intestinal tissues of patients with CD (n = 25) were studied. The gene and protein expression of IL-7, IL-2 receptor subunit γ [cluster of differentiation 132 (CD132)], receptor α (CD127), and caspase-3 in tissues was analyzed by quantitative PCR. Serum IL-7 levels were also analyzed.Results: In patients with CD, a decreased number of γδ T cells and an increase in the apoptosis of CD56+ αβ and γδ T cells in peripheral blood was observed (P < 0.0001 and P < 0.01) respectively, and there was an inverse correlation among T subsets and their apoptosis. In addition, IL-7 gene expression and IL-7 protein in the tissues of these patients were increased. The titers of caspase-3 in tissues were low vs control group (P > 0.01). The percentage of CD8+γδ T cells decreased in tissues (P < 0.01), and was directly related to IL-7 levels in peripheral blood. The expression of IL-2 receptor subunit γ (CD132) was greatly decreased in the tissues of patients with CD (P < 0.05).Conclusion: There may be a cause-effect relationship between the lower gene expression of the IL-2 receptor subunit γ (CD132) in tissues of patients with CD and γδ T cells immunodeficiency.
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