RT Journal Article
T1 Secretome analysis of atherosclerotic and non-atherosclerotic arteries reveals dynamic extracellular remodeling during pathogenesis
A1 Cuesta, Fernando de la
A1 Barderas, Maria
A1 Calvo, Enrique
A1 Zubiri, Irene
A1 Maroto, Aroa
A1 Darde, Veronica
A1 Martin-Rojas, Tatiana
A1 Gil Dones, Félix
A1 Posada-Ayala, Maria
A1 Tejerina, Teresa
A1 Lopez, Juan
A1 Vivanco Martínez, Fernando
A1 Álvarez Llamas, Gloria
AB Aims:Early detection of cardiovascular diseases and knowledge of underlying mechanisms is essential. Tissue secretome studies resemble more closely to the in vivo situation, showing a much narrower protein concentrations dynamic range than plasma. This study was aimed to the analysis of human arterial tissue secretome and to the quantitative comparison of healthy and atherosclerotic secretome to discover proteins with key roles in atherosclerosis development.Methods and results:Secretomes from three biological replicates of human atherosclerotic coronary arteries (APC), preatherosclerotic coronaries (PC) and mammaries (M) were analyzed by LC-MS/MS. The identified proteins were submitted to Ingenuity Pathway Analysis (IPA) tool. Label-free MS/MS based quantification was performed and validated by immunohistochemistry. 64 proteins were identified in the 3 replicates of at least one of the 3 groups and 15 secreted proteins have not been previously reported in plasma. Four proteins were significantly released in higher amounts by mammary tissue: gelsolin, vinculin, lamin A/C and phosphoglucomutase 5.Conclusion:The study of tissue secretome reveals key proteins involved in atherosclerosis which have not been previously reported in plasma. Novel proteins are here highlighted which could be potential therapeutic targets in clinical practice. 
PB Elsevier
SN 1874-3919
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/100398
UL https://hdl.handle.net/20.500.14352/100398
LA eng
NO De La Cuesta, Fernando, et al. «Secretome Analysis of Atherosclerotic and Non-Atherosclerotic Arteries Reveals Dynamic Extracellular Remodeling during Pathogenesis». Journal of Proteomics, vol. 75, n.o 10, junio de 2012, pp. 2960-71. https://doi.org/10.1016/j.jprot.2011.12.005.
NO Ministerio de Educación y Ciencia (España)
NO Comunidad de Madrid
NO Instituto de Salud Carlos III
NO Fundacíon Mutua Madrileña 
NO Red Temática de Investigación Cooperativa en Enfermedades Cardiovasculares 
NO Fundación Jiménez Diaz
DS Docta Complutense
RD 4 abr 2025