RT Journal Article
T1 Genetic engineering as a powerful tool to improve probiotic strains
A1 Landete, José
A1 Bravo Vázquez, Daniel Antonio
AB Over the last decade, there has been increasing interest in the use of probiotic microorganisms. However, certain doubts have arisen around probiotics, because of the beneficial effects of these microorganisms are not clear yet, and in many occasions those beneficial effects have not been proven. Therefore, it would be of interest if these probiotic strains were able to acquire new attributes to allow them improve and increase their beneficial characteristics. Genetic engineering can be used for human applications; for instance, the resistance to antibiotics is removed and the probiotic bacteria are modified in its own DNA. This process can be achieved by (1) the use of food-grade vectors derived from lactic acid bacteria and/or bifidobacteria cryptic plasmids, (2) the genes integration or deletion in the chromosome of the probiotic strain, by site-specific recombination using the attP/integrase system, or by homologous recombination, using either suicide vectors, (3) using the clustered regularly interspaced short palindromic repeats (CRISPR) and the CRISPR-associated (Cas) nuclease. Through genetic engineering, the knowledge of probiotic strains as well as its use could be improved, and the doubts about probiotics could be crumped
PB Taylor & Francis
SN 0264-8725
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/97642
UL https://hdl.handle.net/20.500.14352/97642
LA eng
NO Bravo, D., & Landete, J. M. (2017). Genetic engineering as a powerful tool to improve probiotic strains. Biotechnology and Genetic Engineering Reviews, 33(2), 173–189. https://doi.org/10.1080/02648725.2017.1408257
NO Ministerio de Economía y Competitividad (España)
DS Docta Complutense
RD 1 oct 2024